Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3766005..3766581 Replicon chromosome
Accession NZ_CP043217
Organism Escherichia coli O80:H26 strain EC-107

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag FYA02_RS18790 Protein ID WP_001297643.1
Coordinates 3766005..3766292 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag FYA02_RS18795 Protein ID WP_000063156.1
Coordinates 3766279..3766581 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA02_RS18770 3762258..3763538 - 1281 WP_069722975.1 DUF445 domain-containing protein -
FYA02_RS18775 3763579..3764811 - 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
FYA02_RS18780 3764957..3765052 - 96 Protein_3591 dienelactone hydrolase family protein -
FYA02_RS18785 3765109..3765806 + 698 Protein_3592 IS1 family transposase -
FYA02_RS18790 3766005..3766292 + 288 WP_001297643.1 BrnT family toxin Toxin
FYA02_RS18795 3766279..3766581 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
FYA02_RS18800 3766615..3767571 - 957 WP_001297640.1 GTPase -
FYA02_RS18805 3767582..3767785 - 204 WP_000467859.1 YbdD/YjiX family protein -
FYA02_RS18810 3767835..3769985 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T137956 WP_001297643.1 NZ_CP043217:3766005-3766292 [Escherichia coli O80:H26]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T137956 NZ_CP057151:40058-40216 [Citrobacter sp. RHB35-C17]
ATGAAACTGCCACGAAGTACCCTGATCTGGTGTGTATTAATAGTGTGTCTCACACTGCTGATATTCACTTACCTGACCCG
GAAATCGCTATGCGAAATACGGTACAGGGACACGAACAGGGAGGTGGCGGCTTTTATGGCTTACGAATCCGCTAAGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT137956 WP_000063156.1 NZ_CP043217:3766279-3766581 [Escherichia coli O80:H26]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT137956 NZ_CP057151:39890-40014 [Citrobacter sp. RHB35-C17]
GGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGCAGAAAGCCCCGTAGT
TAATTTTCATTAACCCACGAGGCCACCTGTATGCCTAGACAACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References