Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3505595..3506092 Replicon chromosome
Accession NZ_CP043217
Organism Escherichia coli O80:H26 strain EC-107

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FYA02_RS17375 Protein ID WP_001173343.1
Coordinates 3505919..3506092 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FYA02_RS17370 Protein ID WP_000398619.1
Coordinates 3505595..3505891 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA02_RS17350 3502326..3503045 - 720 WP_000611288.1 two-component system response regulator DcuR -
FYA02_RS17355 3503042..3504673 - 1632 WP_001216477.1 sensor histidine kinase -
FYA02_RS17360 3504854..3505084 + 231 WP_032202296.1 (4Fe-4S)-binding protein -
FYA02_RS17365 3505096..3505368 + 273 WP_000405651.1 N-acetyltransferase -
FYA02_RS17370 3505595..3505891 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FYA02_RS17375 3505919..3506092 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FYA02_RS17380 3506216..3507733 - 1518 WP_001295074.1 lysine--tRNA ligase -
FYA02_RS17385 3507970..3509427 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T137953 WP_001173343.1 NZ_CP043217:3505919-3506092 [Escherichia coli O80:H26]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T137953 NZ_CP057150:c3909223-3908816 [Citrobacter sp. RHB35-C17]
GTGGTCCTGTGGCAATCTGATCTCCGCGTCTCATGGCGCGCACAGTGGCTATCACTCCTTCTGCATGGGCTGGTGGCCGC
GGCTATTCTATTAATGCCATGGCCGCTGAGCTACACGCCGCTGTGGTTGATACTGCTTTCGCTGGTGGTATTTGACTGTG
TCCGCAGCCAGCGGCGAATTAACGCCCGTCAGGGAGAGGTCAAACTTCTGATGGATGGACGACTCCGCTGGCAGGGGCAG
GACTGGCTTATCGTCAGTGCACCGTGGATGATCAAAACCGGCATGATGCTGCGCTTGCGCTCAGAAACCGGTAAACGCCA
GCATTTGTGGCTCGCGGCTGACAGTATGGATAAGGCGGAATGGCGGGATTTACGCCGCCTGATGATGCAACCGTCGACGC
AAGGGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT137953 WP_000398619.1 NZ_CP043217:3505595-3505891 [Escherichia coli O80:H26]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT137953 NZ_CP057150:c3909470-3909204 [Citrobacter sp. RHB35-C17]
ATGGATATTAACAATAAAGCACGTATTCACTGGGCATGCCGACGCGGTATGCGTGAACTCGATATTTCCATCATGCCGTT
TTTCGAACATGAATACGACACGTTGAGTGATGATGAAAAACGTATTTTTATCCGCCTGCTGGAGTGTGATGACCCGGATT
TATTCAACTGGTTGATGAATCACGGTAAACCGGCAGATGCTGAACTGGAGCAGATGGTACGACTCATCCAGACACGGAAT
CGGGATCGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References