Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2150591..2151245 Replicon chromosome
Accession NZ_CP043217
Organism Escherichia coli O80:H26 strain EC-107

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FYA02_RS10915 Protein ID WP_000244777.1
Coordinates 2150591..2150998 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FYA02_RS10920 Protein ID WP_000354046.1
Coordinates 2150979..2151245 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA02_RS10895 2146548..2148281 - 1734 WP_000813208.1 single-stranded-DNA-specific exonuclease RecJ -
FYA02_RS10900 2148287..2148997 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FYA02_RS10905 2149022..2149918 - 897 WP_032202431.1 site-specific tyrosine recombinase XerD -
FYA02_RS10910 2150030..2150551 + 522 WP_001055874.1 flavodoxin FldB -
FYA02_RS10915 2150591..2150998 - 408 WP_000244777.1 toxin CptA Toxin
FYA02_RS10920 2150979..2151245 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FYA02_RS10925 2151488..2152468 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FYA02_RS10930 2152664..2153323 - 660 WP_000250274.1 hemolysin III family protein -
FYA02_RS10935 2153487..2153798 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
FYA02_RS10940 2153843..2155276 + 1434 WP_069722973.1 6-phospho-beta-glucosidase BglA -
FYA02_RS10945 2155333..2156076 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T137949 WP_000244777.1 NZ_CP043217:c2150998-2150591 [Escherichia coli O80:H26]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T137949 NZ_CP057150:1832606-1832863 [Citrobacter sp. RHB35-C17]
ATGAATTTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCCAAAGGTGGACGTATTGCGCACGTCCGCGATGCGTC
AGGCCGCGTCACCGCCGTTGAATGCTACAGCCGCGAAGGGCTGCTGCTCGCCGACTGTACACTTGCCGTCTTCAAAAAAC
TCAAAACCAAAAAGCTGATCAAATCCGTCAATGGTCAGCCGTACCGCATTAATACCACCGGGCTGAACAACGTGCGTGCT
CAGCCAGATAACCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT137949 WP_000354046.1 NZ_CP043217:c2151245-2150979 [Escherichia coli O80:H26]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT137949 NZ_CP043217:c2151245-2150979 [Escherichia coli O80:H26]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References