Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 496507..497033 Replicon chromosome
Accession NZ_CP043217
Organism Escherichia coli O80:H26 strain EC-107

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FYA02_RS02550 Protein ID WP_000323025.1
Coordinates 496507..496794 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FYA02_RS02555 Protein ID WP_000534858.1
Coordinates 496794..497033 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA02_RS02515 492189..493148 - 960 WP_000592549.1 DUF523 and DUF1722 domain-containing protein -
FYA02_RS02520 493341..493865 + 525 WP_148880011.1 outer membrane lipoprotein Blc -
FYA02_RS02525 494021..494398 - 378 WP_001204787.1 antitermination protein -
FYA02_RS02530 494416..495465 - 1050 WP_001265276.1 DUF968 domain-containing protein -
FYA02_RS02535 495467..495745 - 279 WP_023147795.1 hypothetical protein -
FYA02_RS02540 495812..496063 - 252 WP_000980999.1 hypothetical protein -
FYA02_RS02545 496280..496435 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FYA02_RS02550 496507..496794 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FYA02_RS02555 496794..497033 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FYA02_RS02560 497058..497363 + 306 WP_071600497.1 hypothetical protein -
FYA02_RS02565 497566..497652 + 87 Protein_496 protein FlxA -
FYA02_RS02570 497707..498404 + 698 Protein_497 IS1-like element IS1A family transposase -
FYA02_RS02575 498423..498674 + 252 WP_075362600.1 FlxA-like family protein -
FYA02_RS02580 499123..500336 + 1214 WP_181657866.1 IS3 family transposase -
FYA02_RS02590 500424..501764 - 1341 WP_148880013.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 460890..525264 64374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T137938 WP_000323025.1 NZ_CP043217:c496794-496507 [Escherichia coli O80:H26]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T137938 NZ_CP057147:2618868-2619056 [Citrobacter sp. RHB35-C21]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTACCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTGGTTTACAGTCATCATCAATATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT137938 WP_000534858.1 NZ_CP043217:c497033-496794 [Escherichia coli O80:H26]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT137938 NZ_CP043217:c497033-496794 [Escherichia coli O80:H26]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References