Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3508273..3508927 Replicon chromosome
Accession NZ_CP043213
Organism Escherichia coli O16:H48 strain CV601gfp

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FYA04_RS17170 Protein ID WP_000244777.1
Coordinates 3508273..3508680 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FYA04_RS17175 Protein ID WP_000354046.1
Coordinates 3508661..3508927 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA04_RS17150 3504230..3505963 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
FYA04_RS17155 3505969..3506679 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FYA04_RS17160 3506704..3507600 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FYA04_RS17165 3507712..3508233 + 522 WP_001055874.1 flavodoxin FldB -
FYA04_RS17170 3508273..3508680 - 408 WP_000244777.1 toxin CptA Toxin
FYA04_RS17175 3508661..3508927 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FYA04_RS17180 3509170..3510150 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FYA04_RS17185 3510346..3511005 - 660 WP_000250274.1 hemolysin III family protein -
FYA04_RS17190 3511169..3511480 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FYA04_RS17195 3511525..3512958 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
FYA04_RS17200 3513015..3513758 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T137927 WP_000244777.1 NZ_CP043213:c3508680-3508273 [Escherichia coli O16:H48]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T137927 NZ_CP057136:2520073-2520351 [Citrobacter freundii]
ATGATCATGAACTTCAGACATAAAGGGTTGCGCGACTTGTTTCTTCATGGGCGAACATCAGGCGTGACGGCGAAGCATGT
TCGGCGATTGCGGCACCGACTTGCCGTTATTGATGCAGCAAGCCATGTAACAGATATCAATATGCCCGGTTATAAACTGC
ATGCGTTAACGGGCGATCGTGACGGCATTTGGTCGATTTCTGTTTCAGGCAACTGGCGGATTACGTTTGAATTCGTCAAT
GGCGATGCATACATTCTGGATTACGAGGACTATCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT137927 WP_000354046.1 NZ_CP043213:c3508927-3508661 [Escherichia coli O16:H48]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT137927 NZ_CP057136:2520351-2520635 [Citrobacter freundii]
ATGAAAATGGCTAATCATCCCCGTCCGGGGGATATTATTCAGGAAGCGCTGGATGAGTTAAACGTCAGTTTGCGCGAATT
TGCCAGAGCAATGGAAATTGCCCCCTCAACCGCGAGCCGACTGTTAACGGGCAAAGCAGCATTAACCCCGGAAATGGCGA
TCAAACTTTCAGTGGTGATCGGCAGTTCGCCCGAAATGTGGCTGAATCTGCAGAATGCCTGGAGTCTGGCAGAAGCGCAA
AAAAGCGTGGATATCTCACGTCTGCGCAAACTGGTAGCACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References