Detailed information of TA system TA137922

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2549734..2550565
• Replicon: chromosome
• Accession: NZ_CP043213
• Organism: Escherichia coli O16:H48 strain CV601gfp

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: FYA04_RS12695
• Protein ID: WP_000854814.1
• Coordinates: 2550191..2550565 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: FYA04_RS12690
• Protein ID: WP_001285584.1
• Coordinates: 2549734..2550102 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA04_RS12675	2547401..2548933	+	1533	WP_001350525.1	hypothetical protein	-
FYA04_RS12680	2548930..2549376	+	447	WP_000187523.1	RadC family protein	-
FYA04_RS12685	2549439..2549660	+	222	WP_000692323.1	DUF987 domain-containing protein	-
FYA04_RS12690	2549734..2550102	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FYA04_RS12695	2550191..2550565	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FYA04_RS12700	2550562..2550756	+	195	WP_000988600.1	hypothetical protein	-
FYA04_RS12705	2550769..2550882	+	114	WP_001161660.1	DUF957 domain-containing protein	-
FYA04_RS12710	2551171..2551299	-	129	Protein_2476	transposase domain-containing protein	-
FYA04_RS12715	2551371..2551553	+	183	WP_001016348.1	hypothetical protein	-
FYA04_RS12720	2551654..2551983	-	330	WP_000450409.1	DUF496 family protein	-
FYA04_RS12725	2552155..2553213	-	1059	WP_001200891.1	FUSC family protein	-
FYA04_RS12730	2553411..2553884	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
FYA04_RS12735	2554003..2555169	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T137922 WP_000854814.1 NZ_CP043213:2550191-2550565 [Escherichia coli O16:H48]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T137922 NZ_CP043213:2550191-2550565 [Escherichia coli O16:H48]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT137922 WP_001285584.1 NZ_CP043213:2549734-2550102 [Escherichia coli O16:H48]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT137922 NZ_CP043213:2549734-2550102 [Escherichia coli O16:H48]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6