Detailed information of TA system TA137912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2061373..2062961
• Replicon: chromosome
• Accession: NZ_CP043213
• Organism: Escherichia coli O16:H48 strain CV601gfp

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: FYA04_RS10120
• Protein ID: WP_001125439.1
• Coordinates: 2061373..2062695 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FYA04_RS10125
• Protein ID: WP_001301023.1
• Coordinates: 2062695..2062961 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA04_RS10095	2057339..2058253	-	915	WP_000520676.1	fimbrial protein	-
FYA04_RS10100	2058312..2058815	-	504	WP_000825452.1	type 1 fimbrial protein	-
FYA04_RS10105	2058828..2059358	-	531	WP_000876763.1	type 1 fimbrial protein	-
FYA04_RS10110	2059372..2060598	-	1227	Protein_1971	fimbria/pilus outer membrane usher protein	-
FYA04_RS10115	2060853..2061061	-	209	Protein_1972	AraC family transcriptional regulator	-
FYA04_RS10120	2061373..2062695	-	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
FYA04_RS10125	2062695..2062961	-	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
FYA04_RS10130	2063184..2066928	-	3745	Protein_1975	autotransporter barrel domain-containing lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2051361..2069941	18580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T137912 WP_001125439.1 NZ_CP043213:c2062695-2061373 [Escherichia coli O16:H48]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T137912 NZ_CP057125:838769-839176 [Citrobacter sp. RHB36-C18]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCGCAATGGCTTTCGCTGCTCATTCATGGGCTGGTCGCGGT
ATTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTCTGCTCTCTCTGGTGGTGTTCGATAGCG
TTCGTAGCCAGCGCCGAATCAATGCCTGTCAGGGCGAGATTCGGCTGTTGATGGACGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGACTATTGCTAGCGCACCCTGGATGGTCAAAACAGGCATGATGCTGCGTTTACGTTCCGATGCCGGAAAACGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGACGCGGAGTGGCGGGATTTGCGGCGGTTAATACTGCAACAGGCGAAGC
AGGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT137912 WP_001301023.1 NZ_CP043213:c2062961-2062695 [Escherichia coli O16:H48]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT137912 NZ_CP057125:838522-838788 [Citrobacter sp. RHB36-C18]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGATGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGACT
TATTTAACTGGTTGATGAATCATGGGAAACCGGCTGATGCTCAGCTAGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU