Detailed information of TA system TA137899

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 776854..777533
• Replicon: chromosome
• Accession: NZ_CP043213
• Organism: Escherichia coli O16:H48 strain CV601gfp

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FYA04_RS03715
• Protein ID: WP_000854672.1
• Coordinates: 777192..777533 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FYA04_RS03710
• Protein ID: WP_000070395.1
• Coordinates: 776854..777171 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA04_RS03660	771901..772764	+	864	WP_001065553.1	GTPase family protein	-
FYA04_RS03665	772856..773677	+	822	WP_000197389.1	DUF945 domain-containing protein	-
FYA04_RS03670	773894..774085	+	192	Protein_718	DeoR family transcriptional regulator	-
FYA04_RS03675	774081..774308	+	228	WP_001548158.1	hypothetical protein	-
FYA04_RS03680	774308..774751	+	444	WP_000824223.1	hypothetical protein	-
FYA04_RS03685	774774..775241	+	468	WP_001547765.1	hypothetical protein	-
FYA04_RS03690	775318..775557	+	240	WP_000194654.1	DUF905 domain-containing protein	-
FYA04_RS03695	775655..776113	+	459	WP_000211838.1	antirestriction protein	-
FYA04_RS03700	776129..776605	+	477	WP_000811693.1	RadC family protein	-
FYA04_RS03705	776614..776835	+	222	WP_000691994.1	DUF987 domain-containing protein	-
FYA04_RS03710	776854..777171	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FYA04_RS03715	777192..777533	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FYA04_RS03725	778105..779358	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FYA04_RS03730	779370..780473	-	1104	WP_001285288.1	glutamate 5-kinase	-
FYA04_RS03735	780761..781816	+	1056	WP_000749863.1	phosphoporin PhoE	-
FYA04_RS22925	781855..781947	-	93	Protein_731	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	743840..782653	38813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T137899 WP_000854672.1 NZ_CP043213:777192-777533 [Escherichia coli O16:H48]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T137899 NZ_CP043213:777192-777533 [Escherichia coli O16:H48]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT137899 WP_000070395.1 NZ_CP043213:776854-777171 [Escherichia coli O16:H48]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT137899 NZ_CP057104:3315196-3315570 [Escherichia fergusonii]
ATGGACGAATACTCACCAAAACGACATGATATCGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAATCTTGAAGAGAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCTGTCAATCTGCAGCTCAACGAACTGATTGAGC
ATATTGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAGGACAATAAGCTCATAGAGCAGATCGATGAATATCTG
GATGACACTTTTATGTTGTTCAGTAGTTATGGCATTAATACACAGGATTTACAGAAATGGCGAAAATCTGGAAACAGGCT
CTTCCGCTGTTTTGTTAACGCCACCAGGGCGAACCCTGTTAGTTTGTCGTGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684