Detailed information of TA system TA137867

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2089935..2090766
• Replicon: chromosome
• Accession: NZ_CP043211
• Organism: Escherichia coli O16:H48 strain PG20180050

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: FYA05_RS09915
• Protein ID: WP_000854814.1
• Coordinates: 2089935..2090309 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: FYA05_RS09920
• Protein ID: WP_001285584.1
• Coordinates: 2090398..2090766 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA05_RS09875	2085331..2086497	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
FYA05_RS09880	2086616..2087089	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
FYA05_RS09885	2087287..2088345	+	1059	WP_001200891.1	FUSC family protein	-
FYA05_RS09890	2088517..2088846	+	330	WP_000450409.1	DUF496 family protein	-
FYA05_RS09895	2088947..2089129	-	183	WP_001016348.1	hypothetical protein	-
FYA05_RS09900	2089201..2089329	+	129	Protein_1920	transposase domain-containing protein	-
FYA05_RS09905	2089618..2089731	-	114	WP_001161660.1	DUF957 domain-containing protein	-
FYA05_RS09910	2089744..2089938	-	195	WP_000988600.1	hypothetical protein	-
FYA05_RS09915	2089935..2090309	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FYA05_RS09920	2090398..2090766	-	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FYA05_RS09925	2090840..2091061	-	222	WP_000692323.1	DUF987 domain-containing protein	-
FYA05_RS09930	2091124..2091570	-	447	WP_000187523.1	RadC family protein	-
FYA05_RS09935	2091567..2093099	-	1533	WP_001350525.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T137867 WP_000854814.1 NZ_CP043211:c2090309-2089935 [Escherichia coli O16:H48]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T137867 NZ_CP043211:c2090309-2089935 [Escherichia coli O16:H48]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT137867 WP_001285584.1 NZ_CP043211:c2090766-2090398 [Escherichia coli O16:H48]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT137867 NZ_CP043211:c2090766-2090398 [Escherichia coli O16:H48]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6