Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6861..7354 Replicon plasmid pET1.1-IncH
Accession NZ_CP043208
Organism Escherichia coli O16:H48 strain PG20180053

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag FYA07_RS22555 Protein ID WP_001230709.1
Coordinates 6861..7115 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag FYA07_RS22560 Protein ID WP_000151456.1
Coordinates 7112..7354 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA07_RS22515 2433..2594 + 162 WP_001366886.1 type I toxin-antitoxin system Hok family toxin -
FYA07_RS22520 2744..3340 + 597 WP_000125165.1 hypothetical protein -
FYA07_RS23065 3358..3642 - 285 WP_001162371.1 hypothetical protein -
FYA07_RS22530 3716..4381 - 666 WP_000532148.1 P-loop NTPase -
FYA07_RS22540 4970..5299 + 330 WP_000855119.1 hypothetical protein -
FYA07_RS22545 5703..6218 + 516 WP_001025393.1 J domain-containing protein -
FYA07_RS22550 6322..6864 + 543 WP_001083904.1 hypothetical protein -
FYA07_RS22555 6861..7115 + 255 WP_001230709.1 hypothetical protein Toxin
FYA07_RS22560 7112..7354 + 243 WP_000151456.1 hypothetical protein Antitoxin
FYA07_RS22565 7925..8218 + 294 WP_000673428.1 hypothetical protein -
FYA07_RS22570 8628..8810 - 183 WP_024135751.1 hypothetical protein -
FYA07_RS22575 9368..9550 + 183 WP_000833470.1 type II toxin-antitoxin system HicA family toxin -
FYA07_RS22580 9575..10012 + 438 WP_000466318.1 type II toxin-antitoxin system HicB family antitoxin -
FYA07_RS22585 10143..10463 - 321 WP_001401701.1 hypothetical protein -
FYA07_RS22595 10797..10975 - 179 Protein_15 hypothetical protein -
FYA07_RS22600 10977..11180 - 204 WP_000224735.1 hypothetical protein -
FYA07_RS22605 11186..11635 - 450 WP_000206700.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCMY-2 - 1..64997 64997


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-48)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T137822 WP_001230709.1 NZ_CP043208:6861-7115 [Escherichia coli O16:H48]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T137822 NZ_CP043208:6861-7115 [Escherichia coli O16:H48]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT137822 WP_000151456.1 NZ_CP043208:7112-7354 [Escherichia coli O16:H48]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT137822 NZ_CP043208:7112-7354 [Escherichia coli O16:H48]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References