Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4187211..4187708 Replicon chromosome
Accession NZ_CP043201
Organism Escherichia coli O16:H48 strain PG20180059

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FYA10_RS20545 Protein ID WP_001173343.1
Coordinates 4187211..4187384 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FYA10_RS20550 Protein ID WP_000398619.1
Coordinates 4187412..4187708 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA10_RS20535 4183881..4185338 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
FYA10_RS20540 4185575..4187092 + 1518 WP_001295074.1 lysine--tRNA ligase -
FYA10_RS20545 4187211..4187384 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FYA10_RS20550 4187412..4187708 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FYA10_RS20555 4187935..4188207 - 273 WP_000405651.1 N-acetyltransferase -
FYA10_RS20560 4188219..4188449 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
FYA10_RS20565 4188630..4190261 + 1632 WP_001216477.1 sensor histidine kinase -
FYA10_RS20570 4190258..4190977 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T137722 WP_001173343.1 NZ_CP043201:c4187384-4187211 [Escherichia coli O16:H48]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T137722 NZ_CP056910:c4511615-4511331 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACAGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTCGCGGTAGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT137722 WP_000398619.1 NZ_CP043201:c4187708-4187412 [Escherichia coli O16:H48]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT137722 NZ_CP056910:c4511847-4511605 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGATAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGATA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References