Detailed information of TA system TA137715

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3578821..3579500
• Replicon: chromosome
• Accession: NZ_CP043201
• Organism: Escherichia coli O16:H48 strain PG20180059

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FYA10_RS17560
• Protein ID: WP_000854672.1
• Coordinates: 3578821..3579162 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FYA10_RS17565
• Protein ID: WP_000070395.1
• Coordinates: 3579183..3579500 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA10_RS23215	3574407..3574499	+	93	Protein_3418	sigma factor-binding protein Crl	-
FYA10_RS17540	3574538..3575593	-	1056	WP_000749863.1	phosphoporin PhoE	-
FYA10_RS17545	3575881..3576984	+	1104	WP_001285288.1	glutamate 5-kinase	-
FYA10_RS17550	3576996..3578249	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FYA10_RS17560	3578821..3579162	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FYA10_RS17565	3579183..3579500	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FYA10_RS17570	3579519..3579740	-	222	WP_000691994.1	DUF987 domain-containing protein	-
FYA10_RS17575	3579749..3580225	-	477	WP_000811693.1	RadC family protein	-
FYA10_RS17580	3580241..3580699	-	459	WP_000211838.1	antirestriction protein	-
FYA10_RS17585	3580797..3581036	-	240	WP_000194654.1	DUF905 domain-containing protein	-
FYA10_RS17590	3581113..3581580	-	468	WP_001547765.1	hypothetical protein	-
FYA10_RS17595	3581603..3582046	-	444	WP_000824223.1	hypothetical protein	-
FYA10_RS17600	3582046..3582273	-	228	WP_001548158.1	hypothetical protein	-
FYA10_RS17605	3582269..3582460	-	192	Protein_3431	DeoR family transcriptional regulator	-
FYA10_RS17610	3582677..3583498	-	822	WP_000197389.1	DUF945 domain-containing protein	-
FYA10_RS17615	3583590..3584453	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T137715 WP_000854672.1 NZ_CP043201:c3579162-3578821 [Escherichia coli O16:H48]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T137715 NZ_CP056910:2078868-2078971 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT137715 WP_000070395.1 NZ_CP043201:c3579500-3579183 [Escherichia coli O16:H48]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT137715 NZ_CP043201:c3579500-3579183 [Escherichia coli O16:H48]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684