Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4082671..4083325 Replicon chromosome
Accession NZ_CP043199
Organism Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FYA11_RS19885 Protein ID WP_000244777.1
Coordinates 4082671..4083078 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FYA11_RS19890 Protein ID WP_000354046.1
Coordinates 4083059..4083325 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA11_RS19865 4078628..4080361 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
FYA11_RS19870 4080367..4081077 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FYA11_RS19875 4081102..4081998 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FYA11_RS19880 4082110..4082631 + 522 WP_001055874.1 flavodoxin FldB -
FYA11_RS19885 4082671..4083078 - 408 WP_000244777.1 toxin CptA Toxin
FYA11_RS19890 4083059..4083325 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FYA11_RS19895 4083568..4084548 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FYA11_RS19900 4084744..4085403 - 660 WP_000250274.1 hemolysin III family protein -
FYA11_RS19905 4085567..4085878 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FYA11_RS19910 4085923..4087356 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
FYA11_RS19915 4087413..4088156 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T137686 WP_000244777.1 NZ_CP043199:c4083078-4082671 [Escherichia coli O16:H48]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T137686 NZ_CP056899:987076-987483 [Citrobacter sp. RHBSTW-00017]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCGCAATGGCTTTCGTTGCTCATTCATGGGCTGGTCGCGGT
ATTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTCTGCTCTCTCTGGTGGTGTTCGATAGCG
TCCGTAGCCAGCGCCGAATCAATGCCTGTCAGGGCGAGATTCGGCTGTTGATGGACGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGACTATTGCTAGCGCACCCTGGATGGTCAAAACAGGCATGATGTTGCGTTTACGTTCCGATGCCGGAAAACGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGACGCGGAGTGGCGGGATTTGCGGCGGTTAATACTGCAACAGGCGAAGC
AGGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT137686 WP_000354046.1 NZ_CP043199:c4083325-4083059 [Escherichia coli O16:H48]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT137686 NZ_CP056899:986829-987095 [Citrobacter sp. RHBSTW-00017]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGATGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGACT
TATTTAACTGGTTGATGAATCATGGGAAACCGGCTGATGCTCAGCTAGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References