137685

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3953011..3953594 Replicon chromosome
Accession NZ_CP043199
Organism Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag FYA11_RS19310 Protein ID WP_000254738.1
Coordinates 3953011..3953346 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag FYA11_RS19315 Protein ID WP_000581937.1
Coordinates 3953346..3953594 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA11_RS19295 3948898..3950196 - 1299 WP_000036723.1 phosphopyruvate hydratase -
FYA11_RS19300 3950284..3951921 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
FYA11_RS19305 3952149..3952940 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
FYA11_RS19310 3953011..3953346 - 336 WP_000254738.1 endoribonuclease MazF Toxin
FYA11_RS19315 3953346..3953594 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FYA11_RS19320 3953672..3955906 - 2235 WP_000226815.1 GTP pyrophosphokinase -
FYA11_RS19325 3955954..3957255 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T137685 WP_000254738.1 NZ_CP043199:c3953346-3953011 [Escherichia coli O16:H48]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T137685 NZ_CP056899:754747-755088 [Citrobacter sp. RHBSTW-00017]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGTCACCCGTGGCTATCTGGCAAATTCTACTGAC
ACGCCTGCTGGAACAACACTACGGCCTGACCCTGAACGACACGCCGTTCAGGGATGAATCCGTTATACAGGAACATATCG
ATGCAGGTATCACGCTGGCCAATGCCGTTAACTTTCTGGTGGAAAAATACGAGCTTGTTCGTATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT137685 WP_000581937.1 NZ_CP043199:c3953594-3953346 [Escherichia coli O16:H48]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT137685 NZ_CP056899:754409-754726 [Citrobacter sp. RHBSTW-00017]
ATGAGCAACATCACTTGGGGCTTGCAGCGCGATATCACGCCGCTCCTGGGAACACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTACCTAGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCTCCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References