Detailed information of TA system TA137685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3953011..3953594
• Replicon: chromosome
• Accession: NZ_CP043199
• Organism: Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: FYA11_RS19310
• Protein ID: WP_000254738.1
• Coordinates: 3953011..3953346 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: FYA11_RS19315
• Protein ID: WP_000581937.1
• Coordinates: 3953346..3953594 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA11_RS19295	3948898..3950196	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
FYA11_RS19300	3950284..3951921	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
FYA11_RS19305	3952149..3952940	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
FYA11_RS19310	3953011..3953346	-	336	WP_000254738.1	endoribonuclease MazF	Toxin
FYA11_RS19315	3953346..3953594	-	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FYA11_RS19320	3953672..3955906	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
FYA11_RS19325	3955954..3957255	-	1302	WP_000046812.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T137685 WP_000254738.1 NZ_CP043199:c3953346-3953011 [Escherichia coli O16:H48]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T137685 NZ_CP056899:754747-755088 [Citrobacter sp. RHBSTW-00017]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGTCACCCGTGGCTATCTGGCAAATTCTACTGAC
ACGCCTGCTGGAACAACACTACGGCCTGACCCTGAACGACACGCCGTTCAGGGATGAATCCGTTATACAGGAACATATCG
ATGCAGGTATCACGCTGGCCAATGCCGTTAACTTTCTGGTGGAAAAATACGAGCTTGTTCGTATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT137685 WP_000581937.1 NZ_CP043199:c3953594-3953346 [Escherichia coli O16:H48]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT137685 NZ_CP056899:754409-754726 [Citrobacter sp. RHBSTW-00017]
ATGAGCAACATCACTTGGGGCTTGCAGCGCGATATCACGCCGCTCCTGGGAACACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTACCTAGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCTCCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4