Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3112129..3112960 Replicon chromosome
Accession NZ_CP043199
Organism Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag FYA11_RS15315 Protein ID WP_000854814.1
Coordinates 3112586..3112960 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag FYA11_RS15310 Protein ID WP_001285584.1
Coordinates 3112129..3112497 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA11_RS15295 3109796..3111328 + 1533 WP_001350525.1 hypothetical protein -
FYA11_RS15300 3111325..3111771 + 447 WP_000187523.1 RadC family protein -
FYA11_RS15305 3111834..3112055 + 222 WP_000692323.1 DUF987 domain-containing protein -
FYA11_RS15310 3112129..3112497 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
FYA11_RS15315 3112586..3112960 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
FYA11_RS15320 3112957..3113151 + 195 WP_000988600.1 hypothetical protein -
FYA11_RS15325 3113164..3113277 + 114 WP_001161660.1 DUF957 domain-containing protein -
FYA11_RS15330 3113566..3113694 - 129 Protein_2968 transposase domain-containing protein -
FYA11_RS15335 3113766..3113948 + 183 WP_001016348.1 hypothetical protein -
FYA11_RS15340 3114049..3114378 - 330 WP_000450409.1 DUF496 family protein -
FYA11_RS15345 3114550..3115608 - 1059 WP_001200891.1 FUSC family protein -
FYA11_RS15350 3115806..3116279 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
FYA11_RS15355 3116398..3117564 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T137681 WP_000854814.1 NZ_CP043199:3112586-3112960 [Escherichia coli O16:H48]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T137681 NZ_CP056896:c4422854-4422573 [Citrobacter sp. RHBSTW-00021]
ATGACCTATAACCTGGAGTTTTTAGATATTGCGCTCAAGGAGTGGCGCAAGCTTTCCCCTGCATTACGCGAGCAGTTCAA
AAAGAAACTGGCGGAGCGGCTGGAAAATCCCCATGTCCCTGCCGCCCGCCTTTCCGGCAAATTACACCGTTACAAAATCA
AGCTTCGCAGTGCCGGGTATCGTCTGGTCTATCAGGTCGAGGACGAGCAGGTTGTCGTTCTTGTGATCGCAGTGGGCAGA
CGTGATGGTGATGAGGTTTATCATCAGGCTAACCGGCGCTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT137681 WP_001285584.1 NZ_CP043199:3112129-3112497 [Escherichia coli O16:H48]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT137681 NZ_CP043199:3112129-3112497 [Escherichia coli O16:H48]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References