Detailed information of TA system TA137671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2622764..2624352
• Replicon: chromosome
• Accession: NZ_CP043199
• Organism: Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: FYA11_RS12735
• Protein ID: WP_001125439.1
• Coordinates: 2622764..2624086 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: FYA11_RS12740
• Protein ID: WP_001301023.1
• Coordinates: 2624086..2624352 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA11_RS12710	2618730..2619644	-	915	WP_000520676.1	fimbrial protein	-
FYA11_RS12715	2619703..2620206	-	504	WP_000825452.1	type 1 fimbrial protein	-
FYA11_RS12720	2620219..2620749	-	531	WP_000876763.1	type 1 fimbrial protein	-
FYA11_RS12725	2620763..2621989	-	1227	Protein_2462	fimbria/pilus outer membrane usher protein	-
FYA11_RS12730	2622244..2622452	-	209	Protein_2463	AraC family transcriptional regulator	-
FYA11_RS12735	2622764..2624086	-	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
FYA11_RS12740	2624086..2624352	-	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
FYA11_RS12745	2624575..2628319	-	3745	Protein_2466	autotransporter barrel domain-containing lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2612752..2631332	18580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T137671 WP_001125439.1 NZ_CP043199:c2624086-2622764 [Escherichia coli O16:H48]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T137671 NZ_CP056896:716715-717092 [Citrobacter sp. RHBSTW-00021]
ATGCAAACACAACCTGTTCCCCCGACACAGGAGGCTTCACCACGCCCGTCACCAGTGGAGATCTGGCTCCGCCTTTTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGTTAAACGACACGCCATTTTGCAATGATGGTGTGATCCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGCCCTGATACGCACCGATCGTCACGGTTTC
AGCGCAGAAACACAGTCCCCACTCATCAGCAGTATTGATATCCTTCGCGCCCGTAAGGCCTGTGGACTGATGGCACGCAA
TGGCTACAGACTGGTGACCGATATCACCACCGGCAAATACAGCGTGGTGACTCGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT137671 WP_001301023.1 NZ_CP043199:c2624352-2624086 [Escherichia coli O16:H48]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT137671 NZ_CP056896:716339-716665 [Citrobacter sp. RHBSTW-00021]
ATCGCCGAACCCTGGTGGGGGCTTAAACGCGATATCACACCCTGTTTTGGCGCTCGCCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTCGCCGACCGCGCCAGTATCACCGGGCAGTTCAGCGACGCGGATTTACGCCATCTGGACCAGGCATTTCCGC
TGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAATCCCCGCCATCAGCACGGCGTCACGCTCTACGCA
AAAGGGCTGACCTGCAACGCGGATACACTCGGCAGTTGCGGATATGTCTATATAGCGATTTACCCGACACCTGCAACAAC
AGAATAA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU