Detailed information of TA system TA137658

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1338239..1338918
• Replicon: chromosome
• Accession: NZ_CP043199
• Organism: Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FYA11_RS06325
• Protein ID: WP_000854672.1
• Coordinates: 1338577..1338918 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FYA11_RS06320
• Protein ID: WP_000070395.1
• Coordinates: 1338239..1338556 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA11_RS06270	1333286..1334149	+	864	WP_001065553.1	GTPase family protein	-
FYA11_RS06275	1334241..1335062	+	822	WP_000197389.1	DUF945 domain-containing protein	-
FYA11_RS06280	1335279..1335470	+	192	Protein_1210	DeoR family transcriptional regulator	-
FYA11_RS06285	1335466..1335693	+	228	WP_001548158.1	hypothetical protein	-
FYA11_RS06290	1335693..1336136	+	444	WP_000824223.1	hypothetical protein	-
FYA11_RS06295	1336159..1336626	+	468	WP_001547765.1	hypothetical protein	-
FYA11_RS06300	1336703..1336942	+	240	WP_000194654.1	DUF905 domain-containing protein	-
FYA11_RS06305	1337040..1337498	+	459	WP_000211838.1	antirestriction protein	-
FYA11_RS06310	1337514..1337990	+	477	WP_000811693.1	RadC family protein	-
FYA11_RS06315	1337999..1338220	+	222	WP_000691994.1	DUF987 domain-containing protein	-
FYA11_RS06320	1338239..1338556	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FYA11_RS06325	1338577..1338918	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FYA11_RS06335	1339490..1340743	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FYA11_RS06340	1340755..1341858	-	1104	WP_001285288.1	glutamate 5-kinase	-
FYA11_RS06345	1342146..1343201	+	1056	WP_000749863.1	phosphoporin PhoE	-
FYA11_RS23280	1343240..1343332	-	93	Protein_1223	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1305225..1344038	38813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T137658 WP_000854672.1 NZ_CP043199:1338577-1338918 [Escherichia coli O16:H48]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T137658 NZ_CP056883:833598-834008 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT137658 WP_000070395.1 NZ_CP043199:1338239-1338556 [Escherichia coli O16:H48]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT137658 NZ_CP056883:833351-833617 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684