Detailed information of TA system TA137646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 55719..56241
• Replicon: plasmid pPG20180061.1-IncI2
• Accession: NZ_CP043198
• Organism: Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: FYA12_RS22720
• Protein ID: WP_000638823.1
• Coordinates: 55719..56000 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: FYA12_RS22725
• Protein ID: WP_000121741.1
• Coordinates: 55990..56241 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA12_RS22685	51868..52188	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
FYA12_RS22690	52259..52447	-	189	WP_050567133.1	hypothetical protein	-
FYA12_RS22695	52549..53133	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
FYA12_RS22700	53154..53552	-	399	WP_001153665.1	hypothetical protein	-
FYA12_RS22705	53671..54108	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
FYA12_RS22710	54114..55349	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
FYA12_RS22715	55352..55651	-	300	WP_000835764.1	IncN plasmid KikA protein	-
FYA12_RS22720	55719..56000	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FYA12_RS22725	55990..56241	-	252	WP_000121741.1	hypothetical protein	Antitoxin
FYA12_RS22730	56341..56976	-	636	WP_015059536.1	hypothetical protein	-
FYA12_RS22735	57024..57856	+	833	Protein_63	DNA primase	-
FYA12_RS22740	57893..58129	-	237	WP_000750964.1	EexN family lipoprotein	-
FYA12_RS22745	58133..58780	-	648	WP_000653673.1	conjugal transfer protein TrbJ	-
FYA12_RS22750	58786..59772	-	987	WP_001028544.1	type IV secretion system protein	-
FYA12_RS22755	59776..60033	-	258	WP_000739144.1	hypothetical protein	-
FYA12_RS22760	60030..60332	-	303	WP_001360345.1	hypothetical protein	-
FYA12_RS22770	60603..61049	-	447	WP_001243164.1	hypothetical protein	-
FYA12_RS22915	61060..61230	-	171	WP_000550720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-2	-	1..64997	64997

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T137646 WP_000638823.1 NZ_CP043198:c56000-55719 [Escherichia coli O16:H48]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T137646 NZ_CP043198:c56000-55719 [Escherichia coli O16:H48]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT137646 WP_000121741.1 NZ_CP043198:c56241-55990 [Escherichia coli O16:H48]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT137646 NZ_CP043198:c56241-55990 [Escherichia coli O16:H48]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1