Detailed information of TA system TA137645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 6861..7354
• Replicon: plasmid pPG20180061.1-IncI2
• Accession: NZ_CP043198
• Organism: Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: FYA12_RS22410
• Protein ID: WP_001230709.1
• Coordinates: 6861..7115 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: FYA12_RS22415
• Protein ID: WP_000151456.1
• Coordinates: 7112..7354 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA12_RS22370	2433..2594	+	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
FYA12_RS22375	2744..3340	+	597	WP_000125165.1	hypothetical protein	-
FYA12_RS22900	3358..3642	-	285	WP_001162371.1	hypothetical protein	-
FYA12_RS22385	3716..4381	-	666	WP_000532148.1	P-loop NTPase	-
FYA12_RS22395	4970..5299	+	330	WP_000855119.1	hypothetical protein	-
FYA12_RS22400	5703..6218	+	516	WP_001025393.1	J domain-containing protein	-
FYA12_RS22405	6322..6864	+	543	WP_001083904.1	hypothetical protein	-
FYA12_RS22410	6861..7115	+	255	WP_001230709.1	hypothetical protein	Toxin
FYA12_RS22415	7112..7354	+	243	WP_000151456.1	hypothetical protein	Antitoxin
FYA12_RS22420	7925..8218	+	294	WP_000673428.1	hypothetical protein	-
FYA12_RS22425	8628..8810	-	183	WP_024135751.1	hypothetical protein	-
FYA12_RS22430	9368..9550	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
FYA12_RS22435	9575..10012	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
FYA12_RS22440	10143..10463	-	321	WP_001401701.1	hypothetical protein	-
FYA12_RS22450	10797..10975	-	179	Protein_15	hypothetical protein	-
FYA12_RS22455	10977..11180	-	204	WP_000224735.1	hypothetical protein	-
FYA12_RS22460	11186..11635	-	450	WP_000206700.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-2	-	1..64997	64997

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T137645 WP_001230709.1 NZ_CP043198:6861-7115 [Escherichia coli O16:H48]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T137645 NZ_CP056878:1787630-1787962 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCCACCAGCGGCCATGAACAAAGCGGAAAACGTCCGGTACTCAT
CGTGTCGAAGGCATCATTTAACAAGCTGACCCAGCTACCCGTTATTGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGAATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT137645 WP_000151456.1 NZ_CP043198:7112-7354 [Escherichia coli O16:H48]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT137645 NZ_CP043198:7112-7354 [Escherichia coli O16:H48]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5