Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2956072..2956655 Replicon chromosome
Accession NZ_CP043197
Organism Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag FYA12_RS14335 Protein ID WP_000254738.1
Coordinates 2956320..2956655 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag FYA12_RS14330 Protein ID WP_000581937.1
Coordinates 2956072..2956320 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA12_RS14320 2952411..2953712 + 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
FYA12_RS14325 2953760..2955994 + 2235 WP_000226815.1 GTP pyrophosphokinase -
FYA12_RS14330 2956072..2956320 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FYA12_RS14335 2956320..2956655 + 336 WP_000254738.1 endoribonuclease MazF Toxin
FYA12_RS14340 2956726..2957517 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
FYA12_RS14345 2957745..2959382 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
FYA12_RS14350 2959470..2960768 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T137629 WP_000254738.1 NZ_CP043197:2956320-2956655 [Escherichia coli O16:H48]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T137629 NZ_CP056854:659978-660355 [Klebsiella pneumoniae]
ATGCAAACCCAACCACTATCCTCAACTCAGGAGGCTACACCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACACTCAACGACACTCCGTTCGGCAACGATGGCGTGATTCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGATCTGGTACGAACCGACCGTCGCGGTTTT
AACGCAGAAACACAGTCGCCTCTTCTCAGCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGACTGATGACCCGAAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGGGAGGTGCAGCCATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT137629 WP_000581937.1 NZ_CP043197:2956072-2956320 [Escherichia coli O16:H48]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT137629 NZ_CP043197:2956072-2956320 [Escherichia coli O16:H48]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References