Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2826341..2826995 Replicon chromosome
Accession NZ_CP043197
Organism Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FYA12_RS13760 Protein ID WP_000244777.1
Coordinates 2826588..2826995 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FYA12_RS13755 Protein ID WP_000354046.1
Coordinates 2826341..2826607 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA12_RS13730 2821510..2822253 + 744 WP_000951964.1 SDR family oxidoreductase -
FYA12_RS13735 2822310..2823743 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
FYA12_RS13740 2823788..2824099 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FYA12_RS13745 2824263..2824922 + 660 WP_000250274.1 hemolysin III family protein -
FYA12_RS13750 2825118..2826098 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FYA12_RS13755 2826341..2826607 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FYA12_RS13760 2826588..2826995 + 408 WP_000244777.1 toxin CptA Toxin
FYA12_RS13765 2827035..2827556 - 522 WP_001055874.1 flavodoxin FldB -
FYA12_RS13770 2827668..2828564 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FYA12_RS13775 2828589..2829299 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FYA12_RS13780 2829305..2831038 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T137628 WP_000244777.1 NZ_CP043197:2826588-2826995 [Escherichia coli O16:H48]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T137628 NZ_CP056854:331778-332146 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGTCTGGCGTTGCCGG
TATGCCCGATCTGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT137628 WP_000354046.1 NZ_CP043197:2826341-2826607 [Escherichia coli O16:H48]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT137628 NZ_CP056854:331560-331781 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References