Detailed information of TA system TA137622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 1329415..1330229
• Replicon: chromosome
• Accession: NZ_CP043197
• Organism: Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: FYA12_RS06585
• Protein ID: WP_001054376.1
• Coordinates: 1329415..1329672 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: FYA12_RS06590
• Protein ID: WP_001309181.1
• Coordinates: 1329684..1330229 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA12_RS06565	1324703..1325809	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
FYA12_RS06570	1325874..1326854	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
FYA12_RS06575	1327437..1328677	-	1241	Protein_1278	helicase YjhR	-
FYA12_RS06580	1328793..1329038	+	246	Protein_1279	GNAT family N-acetyltransferase	-
FYA12_RS06585	1329415..1329672	+	258	WP_001054376.1	hypothetical protein	Toxin
FYA12_RS06590	1329684..1330229	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
FYA12_RS06595	1330285..1331031	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
FYA12_RS06600	1331200..1331418	+	219	Protein_1283	hypothetical protein	-
FYA12_RS22950	1331456..1331572	+	117	Protein_1284	VOC family protein	-
FYA12_RS06605	1331817..1332938	+	1122	WP_000010829.1	M42 family metallopeptidase	-
FYA12_RS06610	1332935..1333213	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
FYA12_RS06615	1333225..1334538	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	1320666..1338453	17787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T137622 WP_001054376.1 NZ_CP043197:1329415-1329672 [Escherichia coli O16:H48]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T137622 NZ_CP043197:1329415-1329672 [Escherichia coli O16:H48]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT137622 WP_001309181.1 NZ_CP043197:1329684-1330229 [Escherichia coli O16:H48]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT137622 NZ_CP043197:1329684-1330229 [Escherichia coli O16:H48]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8