Detailed information of TA system TA137617

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 901997..902676
• Replicon: chromosome
• Accession: NZ_CP043197
• Organism: Escherichia coli O16:H48 strain PG20180061

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FYA12_RS04515
• Protein ID: WP_000854672.1
• Coordinates: 901997..902338 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FYA12_RS04520
• Protein ID: WP_000070395.1
• Coordinates: 902359..902676 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA12_RS22820	897583..897675	+	93	Protein_870	sigma factor-binding protein Crl	-
FYA12_RS04495	897714..898769	-	1056	WP_000749863.1	phosphoporin PhoE	-
FYA12_RS04500	899057..900160	+	1104	WP_001285288.1	glutamate 5-kinase	-
FYA12_RS04505	900172..901425	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FYA12_RS04515	901997..902338	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FYA12_RS04520	902359..902676	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FYA12_RS04525	902695..902916	-	222	WP_000691994.1	DUF987 domain-containing protein	-
FYA12_RS04530	902925..903401	-	477	WP_000811693.1	RadC family protein	-
FYA12_RS04535	903417..903875	-	459	WP_000211838.1	antirestriction protein	-
FYA12_RS04540	903973..904212	-	240	WP_000194654.1	DUF905 domain-containing protein	-
FYA12_RS04545	904289..904756	-	468	WP_001547765.1	hypothetical protein	-
FYA12_RS04550	904779..905222	-	444	WP_000824223.1	hypothetical protein	-
FYA12_RS04555	905222..905449	-	228	WP_001548158.1	hypothetical protein	-
FYA12_RS04560	905445..905636	-	192	Protein_883	DeoR family transcriptional regulator	-
FYA12_RS04565	905853..906674	-	822	WP_000197389.1	DUF945 domain-containing protein	-
FYA12_RS04570	906766..907629	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	896877..935690	38813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T137617 WP_000854672.1 NZ_CP043197:c902338-901997 [Escherichia coli O16:H48]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T137617 NZ_CP056852:259064-259432 [Citrobacter freundii]
ATGACGCTACAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTGACGCCTGGTGTCGCAGG
TATGCCTGACCCCGGTCGTGCGGAAGCGCTGATGTATCGGGTATTGAATCAAATCGAATATGAAGGTGTGACTGACGTGT
GGCTACTGGCGGCAATGCATTTGTTAGCCATTTCCCGTGGGCATATCTTTAATGATGGTAACAAGCGTACTGCGTTATTT
ATTACGTTGCTGTTTTTAAAACGTAATGGGATCGTATTATCTGCAAACCCTGATTTTGTCGCCATGACGGTTGAAGCGGC
AGCAGGACAACTCACGTTAGAACAAATTGCGCATCGTCTGCGCCATTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT137617 WP_000070395.1 NZ_CP043197:c902676-902359 [Escherichia coli O16:H48]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT137617 NZ_CP043197:c902676-902359 [Escherichia coli O16:H48]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684