137615

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 37725..38218 Replicon plasmid pPG20180062.1-IncI2
Accession NZ_CP043196
Organism Escherichia coli O16:H48 strain PG20180062

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag FYA13_RS22960 Protein ID WP_001230709.1
Coordinates 37725..37979 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag FYA13_RS22965 Protein ID WP_000151456.1
Coordinates 37976..38218 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA13_RS22920 33297..33458 + 162 WP_001366886.1 type I toxin-antitoxin system Hok family toxin -
FYA13_RS22925 33608..34204 + 597 WP_000125165.1 hypothetical protein -
FYA13_RS23250 34222..34506 - 285 WP_001162371.1 hypothetical protein -
FYA13_RS22935 34580..35245 - 666 WP_000532148.1 P-loop NTPase -
FYA13_RS22945 35834..36163 + 330 WP_000855119.1 hypothetical protein -
FYA13_RS22950 36567..37082 + 516 WP_001025393.1 J domain-containing protein -
FYA13_RS22955 37186..37728 + 543 WP_001083904.1 hypothetical protein -
FYA13_RS22960 37725..37979 + 255 WP_001230709.1 hypothetical protein Toxin
FYA13_RS22965 37976..38218 + 243 WP_000151456.1 hypothetical protein Antitoxin
FYA13_RS22970 38789..39082 + 294 WP_000673428.1 hypothetical protein -
FYA13_RS22975 39492..39674 - 183 WP_024135751.1 hypothetical protein -
FYA13_RS22980 40232..40414 + 183 WP_000833470.1 type II toxin-antitoxin system HicA family toxin -
FYA13_RS22985 40439..40876 + 438 WP_000466318.1 type II toxin-antitoxin system HicB family antitoxin -
FYA13_RS22990 41007..41327 - 321 WP_001401701.1 hypothetical protein -
FYA13_RS23000 41661..41839 - 179 Protein_54 hypothetical protein -
FYA13_RS23005 41841..42044 - 204 WP_000224735.1 hypothetical protein -
FYA13_RS23010 42050..42499 - 450 WP_000206700.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCMY-2 - 1..64786 64786


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T137615 WP_001230709.1 NZ_CP043196:37725-37979 [Escherichia coli O16:H48]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T137615 NZ_CP056850:3841796-3842014 [Citrobacter freundii]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGTCTTCGACGCTGCCAGACAATTGACACGCTCGAGCGCGT
TATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACGATGAATAAGCTGTACGACAAGATTCCAACTTCCGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT137615 WP_000151456.1 NZ_CP043196:37976-38218 [Escherichia coli O16:H48]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT137615 NZ_CP043196:37976-38218 [Escherichia coli O16:H48]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References