Detailed information of TA system TA137606

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3867064..3867743
• Replicon: chromosome
• Accession: NZ_CP043195
• Organism: Escherichia coli O16:H48 strain PG20180062

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FYA13_RS18910
• Protein ID: WP_000854672.1
• Coordinates: 3867064..3867405 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FYA13_RS18915
• Protein ID: WP_000070395.1
• Coordinates: 3867426..3867743 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA13_RS23210	3862650..3862742	+	93	Protein_3665	sigma factor-binding protein Crl	-
FYA13_RS18890	3862781..3863836	-	1056	WP_000749863.1	phosphoporin PhoE	-
FYA13_RS18895	3864124..3865227	+	1104	WP_001285288.1	glutamate 5-kinase	-
FYA13_RS18900	3865239..3866492	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FYA13_RS18910	3867064..3867405	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FYA13_RS18915	3867426..3867743	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FYA13_RS18920	3867762..3867983	-	222	WP_000691994.1	DUF987 domain-containing protein	-
FYA13_RS18925	3867992..3868468	-	477	WP_000811693.1	RadC family protein	-
FYA13_RS18930	3868484..3868942	-	459	WP_000211838.1	antirestriction protein	-
FYA13_RS18935	3869040..3869279	-	240	WP_000194654.1	DUF905 domain-containing protein	-
FYA13_RS18940	3869356..3869823	-	468	WP_001547765.1	hypothetical protein	-
FYA13_RS18945	3869846..3870289	-	444	WP_000824223.1	hypothetical protein	-
FYA13_RS18950	3870289..3870516	-	228	WP_001548158.1	hypothetical protein	-
FYA13_RS18955	3870512..3870703	-	192	Protein_3678	DeoR family transcriptional regulator	-
FYA13_RS18960	3870920..3871741	-	822	WP_000197389.1	DUF945 domain-containing protein	-
FYA13_RS18965	3871833..3872696	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T137606 WP_000854672.1 NZ_CP043195:c3867405-3867064 [Escherichia coli O16:H48]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T137606 NZ_CP043195:c3867405-3867064 [Escherichia coli O16:H48]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT137606 WP_000070395.1 NZ_CP043195:c3867743-3867426 [Escherichia coli O16:H48]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT137606 NZ_CP043195:c3867743-3867426 [Escherichia coli O16:H48]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684