137604

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2664147..2664785 Replicon chromosome
Accession NZ_CP043195
Organism Escherichia coli O16:H48 strain PG20180062

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FYA13_RS12860 Protein ID WP_000813794.1
Coordinates 2664609..2664785 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FYA13_RS12855 Protein ID WP_001270286.1
Coordinates 2664147..2664563 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA13_RS12835 2659299..2660240 - 942 WP_001251304.1 ABC transporter permease -
FYA13_RS12840 2660241..2661254 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FYA13_RS12845 2661272..2662417 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FYA13_RS12850 2662662..2664068 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
FYA13_RS12855 2664147..2664563 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FYA13_RS12860 2664609..2664785 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FYA13_RS12865 2665007..2665237 + 231 WP_000494244.1 YncJ family protein -
FYA13_RS12870 2665329..2667290 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FYA13_RS12875 2667363..2667899 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FYA13_RS12880 2667952..2669166 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T137604 WP_000813794.1 NZ_CP043195:c2664785-2664609 [Escherichia coli O16:H48]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T137604 NZ_CP056841:1726-2010 [Citrobacter freundii]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT137604 WP_001270286.1 NZ_CP043195:c2664563-2664147 [Escherichia coli O16:H48]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT137604 NZ_CP043195:c2664563-2664147 [Escherichia coli O16:H48]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References