Detailed information of TA system TA137580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4006423..4007237
• Replicon: chromosome
• Accession: NZ_CP043193
• Organism: Escherichia coli O16:H48 strain PG20180063

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: FYA14_RS19645
• Protein ID: WP_001054376.1
• Coordinates: 4006423..4006680 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: FYA14_RS19650
• Protein ID: WP_001309181.1
• Coordinates: 4006692..4007237 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA14_RS19625	4001711..4002817	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
FYA14_RS19630	4002882..4003862	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
FYA14_RS19635	4004445..4005685	-	1241	Protein_3826	helicase YjhR	-
FYA14_RS19640	4005801..4006046	+	246	Protein_3827	GNAT family N-acetyltransferase	-
FYA14_RS19645	4006423..4006680	+	258	WP_001054376.1	hypothetical protein	Toxin
FYA14_RS19650	4006692..4007237	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
FYA14_RS19655	4007293..4008039	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
FYA14_RS19660	4008208..4008426	+	219	Protein_3831	hypothetical protein	-
FYA14_RS23420	4008464..4008580	+	117	Protein_3832	VOC family protein	-
FYA14_RS19665	4008825..4009946	+	1122	WP_000010829.1	M42 family metallopeptidase	-
FYA14_RS19670	4009943..4010221	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
FYA14_RS19675	4010233..4011546	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	3995433..4015461	20028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T137580 WP_001054376.1 NZ_CP043193:4006423-4006680 [Escherichia coli O16:H48]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T137580 NZ_CP043193:4006423-4006680 [Escherichia coli O16:H48]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT137580 WP_001309181.1 NZ_CP043193:4006692-4007237 [Escherichia coli O16:H48]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT137580 NZ_CP043193:4006692-4007237 [Escherichia coli O16:H48]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8