Detailed information of TA system TA137573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2376088..2376726
• Replicon: chromosome
• Accession: NZ_CP043193
• Organism: Escherichia coli O16:H48 strain PG20180063

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FYA14_RS11525
• Protein ID: WP_000813794.1
• Coordinates: 2376550..2376726 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FYA14_RS11520
• Protein ID: WP_001270286.1
• Coordinates: 2376088..2376504 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA14_RS11500	2371240..2372181	-	942	WP_001251304.1	ABC transporter permease	-
FYA14_RS11505	2372182..2373195	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
FYA14_RS11510	2373213..2374358	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
FYA14_RS11515	2374603..2376009	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
FYA14_RS11520	2376088..2376504	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FYA14_RS11525	2376550..2376726	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FYA14_RS11530	2376948..2377178	+	231	WP_000494244.1	YncJ family protein	-
FYA14_RS11535	2377270..2379231	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FYA14_RS11540	2379304..2379840	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
FYA14_RS11545	2379893..2381107	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T137573 WP_000813794.1 NZ_CP043193:c2376726-2376550 [Escherichia coli O16:H48]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T137573 NZ_CP056830:c4643408-4642875 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT137573 WP_001270286.1 NZ_CP043193:c2376504-2376088 [Escherichia coli O16:H48]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT137573 NZ_CP043193:c2376504-2376088 [Escherichia coli O16:H48]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB