Detailed information of TA system TA137545

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3579002..3579681
• Replicon: chromosome
• Accession: NZ_CP043191
• Organism: Escherichia coli O16:H48 strain PG20180173

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FYA15_RS17565
• Protein ID: WP_000854672.1
• Coordinates: 3579002..3579343 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FYA15_RS17570
• Protein ID: WP_000070395.1
• Coordinates: 3579364..3579681 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA15_RS23840	3574588..3574680	+	93	Protein_3419	sigma factor-binding protein Crl	-
FYA15_RS17545	3574719..3575774	-	1056	WP_000749863.1	phosphoporin PhoE	-
FYA15_RS17550	3576062..3577165	+	1104	WP_001285288.1	glutamate 5-kinase	-
FYA15_RS17555	3577177..3578430	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FYA15_RS17565	3579002..3579343	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FYA15_RS17570	3579364..3579681	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FYA15_RS17575	3579700..3579921	-	222	WP_000691994.1	DUF987 domain-containing protein	-
FYA15_RS17580	3579930..3580406	-	477	WP_000811693.1	RadC family protein	-
FYA15_RS17585	3580422..3580880	-	459	WP_000211838.1	antirestriction protein	-
FYA15_RS17590	3580978..3581217	-	240	WP_000194654.1	DUF905 domain-containing protein	-
FYA15_RS17595	3581294..3581761	-	468	WP_001547765.1	hypothetical protein	-
FYA15_RS17600	3581784..3582227	-	444	WP_000824223.1	hypothetical protein	-
FYA15_RS17605	3582227..3582454	-	228	WP_001548158.1	hypothetical protein	-
FYA15_RS17610	3582450..3582641	-	192	Protein_3432	DeoR family transcriptional regulator	-
FYA15_RS17615	3582858..3583679	-	822	WP_000197389.1	DUF945 domain-containing protein	-
FYA15_RS17620	3583771..3584634	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T137545 WP_000854672.1 NZ_CP043191:c3579343-3579002 [Escherichia coli O16:H48]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T137545 NZ_CP056779:2860654-2860965 [Enterobacter cloacae]
ATGCTATTCATTGAAACGGATATCTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTTCAGGT
TTTTCTGGCAACGCAACCTGATTATGGCGATCTGATCCAGAATACGGGAGGCCTGCGAAAGATTCGCTGGCTTTCAGGGG
GCCGGGGAAAGCGTGGTGGAGTAAGGATTATTTATTTTCACCGTACGCGCGAGTTTGAAATCAGGCTGCTGCTTATCTAC
CGCAAAGGGATCAAAGATGACCTGTCCGCGGGGGAGAAAGCCATTCTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT137545 WP_000070395.1 NZ_CP043191:c3579681-3579364 [Escherichia coli O16:H48]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT137545 NZ_CP043191:c3579681-3579364 [Escherichia coli O16:H48]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684