Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1804957..1805788 Replicon chromosome
Accession NZ_CP043187
Organism Escherichia coli O16:H48 strain PG20180170

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag FYA17_RS08585 Protein ID WP_000854814.1
Coordinates 1804957..1805331 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag FYA17_RS08590 Protein ID WP_001285584.1
Coordinates 1805420..1805788 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA17_RS08545 1800353..1801519 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
FYA17_RS08550 1801638..1802111 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
FYA17_RS08555 1802309..1803367 + 1059 WP_001200891.1 FUSC family protein -
FYA17_RS08560 1803539..1803868 + 330 WP_000450409.1 DUF496 family protein -
FYA17_RS08565 1803969..1804151 - 183 WP_001016348.1 hypothetical protein -
FYA17_RS08570 1804223..1804351 + 129 Protein_1673 transposase domain-containing protein -
FYA17_RS08575 1804640..1804753 - 114 WP_001161660.1 DUF957 domain-containing protein -
FYA17_RS08580 1804766..1804960 - 195 WP_000988600.1 hypothetical protein -
FYA17_RS08585 1804957..1805331 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
FYA17_RS08590 1805420..1805788 - 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
FYA17_RS08595 1805862..1806083 - 222 WP_000692323.1 DUF987 domain-containing protein -
FYA17_RS08600 1806146..1806592 - 447 WP_000187523.1 RadC family protein -
FYA17_RS08605 1806589..1808121 - 1533 WP_001350525.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T137469 WP_000854814.1 NZ_CP043187:c1805331-1804957 [Escherichia coli O16:H48]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T137469 NZ_CP043187:c1805331-1804957 [Escherichia coli O16:H48]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT137469 WP_001285584.1 NZ_CP043187:c1805788-1805420 [Escherichia coli O16:H48]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT137469 NZ_CP056747:4635518-4635820 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References