Detailed information of TA system TA137457

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3691339..3692018
• Replicon: chromosome
• Accession: NZ_CP043185
• Organism: Escherichia coli O16:H48 strain PG20180171

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: FYA18_RS17830
• Protein ID: WP_000854672.1
• Coordinates: 3691677..3692018 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: FYA18_RS17825
• Protein ID: WP_000070395.1
• Coordinates: 3691339..3691656 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA18_RS17775	3686386..3687249	+	864	WP_001065553.1	GTPase family protein	-
FYA18_RS17780	3687341..3688162	+	822	WP_000197389.1	DUF945 domain-containing protein	-
FYA18_RS17785	3688379..3688570	+	192	Protein_3455	DeoR family transcriptional regulator	-
FYA18_RS17790	3688566..3688793	+	228	WP_001548158.1	hypothetical protein	-
FYA18_RS17795	3688793..3689236	+	444	WP_000824223.1	hypothetical protein	-
FYA18_RS17800	3689259..3689726	+	468	WP_001547765.1	hypothetical protein	-
FYA18_RS17805	3689803..3690042	+	240	WP_000194654.1	DUF905 domain-containing protein	-
FYA18_RS17810	3690140..3690598	+	459	WP_000211838.1	antirestriction protein	-
FYA18_RS17815	3690614..3691090	+	477	WP_000811693.1	RadC family protein	-
FYA18_RS17820	3691099..3691320	+	222	WP_000691994.1	DUF987 domain-containing protein	-
FYA18_RS17825	3691339..3691656	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
FYA18_RS17830	3691677..3692018	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
FYA18_RS17840	3692590..3693843	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
FYA18_RS17845	3693855..3694958	-	1104	WP_001285288.1	glutamate 5-kinase	-
FYA18_RS17850	3695246..3696301	+	1056	WP_000749863.1	phosphoporin PhoE	-
FYA18_RS22685	3696340..3696432	-	93	Protein_3468	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3672160..3697138	24978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T137457 WP_000854672.1 NZ_CP043185:3691677-3692018 [Escherichia coli O16:H48]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T137457 NZ_CP043185:3691677-3692018 [Escherichia coli O16:H48]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT137457 WP_000070395.1 NZ_CP043185:3691339-3691656 [Escherichia coli O16:H48]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT137457 NZ_CP056747:c97581-97282 [Enterobacter hormaechei]
GTGAAAACCTTAAATGAAGTGATTGCGTCACGCTCACCGGAAAGCCAACAGCGCATTCAGGAGATGGCAGAGGAAATGTT
CCTCGAAACCGGTCTGCAACTTCTGCGTGAAGAGCTCCAGCTTTCGCAAAAACATGTCGCCGATCTTATGGGCGTAAGTC
AACCTGCGGTAACACAGCTGGAGCAGCGTGGAAACGATCTTAAGATTGCTACGCTTAAGCGTTACATCGAAGCGATGGGC
GGAAAACTTAGCATTAACGTGGAACTTCCCACTGGCAAGCGGGTTGCGTTTCATCTCTAG

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684