Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 808478..809309 Replicon chromosome
Accession NZ_CP043185
Organism Escherichia coli O16:H48 strain PG20180171

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag FYA18_RS04120 Protein ID WP_000854814.1
Coordinates 808935..809309 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag FYA18_RS04115 Protein ID WP_001285584.1
Coordinates 808478..808846 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA18_RS04100 806145..807677 + 1533 WP_001350525.1 hypothetical protein -
FYA18_RS04105 807674..808120 + 447 WP_000187523.1 RadC family protein -
FYA18_RS04110 808183..808404 + 222 WP_000692323.1 DUF987 domain-containing protein -
FYA18_RS04115 808478..808846 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
FYA18_RS04120 808935..809309 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
FYA18_RS04125 809306..809500 + 195 WP_000988600.1 hypothetical protein -
FYA18_RS04130 809513..809626 + 114 WP_001161660.1 DUF957 domain-containing protein -
FYA18_RS04135 809915..810043 - 129 Protein_806 transposase domain-containing protein -
FYA18_RS04140 810115..810297 + 183 WP_001016348.1 hypothetical protein -
FYA18_RS04145 810398..810727 - 330 WP_000450409.1 DUF496 family protein -
FYA18_RS04150 810899..811957 - 1059 WP_001200891.1 FUSC family protein -
FYA18_RS04155 812155..812628 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
FYA18_RS04160 812747..813913 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T137437 WP_000854814.1 NZ_CP043185:808935-809309 [Escherichia coli O16:H48]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T137437 NZ_CP043185:808935-809309 [Escherichia coli O16:H48]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT137437 WP_001285584.1 NZ_CP043185:808478-808846 [Escherichia coli O16:H48]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT137437 NZ_CP043185:808478-808846 [Escherichia coli O16:H48]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References