Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 237545..238183 Replicon chromosome
Accession NZ_CP043185
Organism Escherichia coli O16:H48 strain PG20180171

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FYA18_RS01180 Protein ID WP_000813794.1
Coordinates 237545..237721 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FYA18_RS01185 Protein ID WP_001270286.1
Coordinates 237767..238183 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA18_RS01160 233164..234378 - 1215 WP_001326689.1 BenE family transporter YdcO -
FYA18_RS01165 234431..234967 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FYA18_RS01170 235040..237001 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FYA18_RS01175 237093..237323 - 231 WP_000494244.1 YncJ family protein -
FYA18_RS01180 237545..237721 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FYA18_RS01185 237767..238183 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FYA18_RS01190 238262..239668 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
FYA18_RS01195 239913..241058 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FYA18_RS01200 241076..242089 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FYA18_RS01205 242090..243031 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T137426 WP_000813794.1 NZ_CP043185:237545-237721 [Escherichia coli O16:H48]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T137426 NZ_CP056737:970762-971130 [Enterobacter roggenkampii]
ATGAAAACTCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCTCCAGTGGCTGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATCAAACCGTTATACAGGAACATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAAAAGTATGAGTTAGTACGTATCGATCGCACAGGATTT
ACCTCACAGGAACAAGCGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGCATGCGGATTAATGAACAGTTG
TTCGTATCGGGAAGTAAGTAATATTGTTCTTAGCCGATCGCGGCAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT137426 WP_001270286.1 NZ_CP043185:237767-238183 [Escherichia coli O16:H48]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT137426 NZ_CP043185:237767-238183 [Escherichia coli O16:H48]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References