Detailed information of TA system TA137412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2376087..2376725
• Replicon: chromosome
• Accession: NZ_CP043183
• Organism: Escherichia coli O16:H48 strain PG20180172

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FYA19_RS11525
• Protein ID: WP_000813794.1
• Coordinates: 2376549..2376725 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FYA19_RS11520
• Protein ID: WP_001270286.1
• Coordinates: 2376087..2376503 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA19_RS11500	2371239..2372180	-	942	WP_001251304.1	ABC transporter permease	-
FYA19_RS11505	2372181..2373194	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
FYA19_RS11510	2373212..2374357	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
FYA19_RS11515	2374602..2376008	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
FYA19_RS11520	2376087..2376503	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FYA19_RS11525	2376549..2376725	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FYA19_RS11530	2376947..2377177	+	231	WP_000494244.1	YncJ family protein	-
FYA19_RS11535	2377269..2379230	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FYA19_RS11540	2379303..2379839	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
FYA19_RS11545	2379892..2381106	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T137412 WP_000813794.1 NZ_CP043183:c2376725-2376549 [Escherichia coli O16:H48]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T137412 NZ_CP043183:c2376725-2376549 [Escherichia coli O16:H48]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT137412 WP_001270286.1 NZ_CP043183:c2376503-2376087 [Escherichia coli O16:H48]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT137412 NZ_CP056721:c34758-34528 [Enterobacter hormaechei]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Structures

Toxin

• PDB: 6HPB