Detailed information of TA system TA137401

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1804961..1805792
• Replicon: chromosome
• Accession: NZ_CP043183
• Organism: Escherichia coli O16:H48 strain PG20180172

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: FYA19_RS08585
• Protein ID: WP_000854814.1
• Coordinates: 1804961..1805335 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: FYA19_RS08590
• Protein ID: WP_001285584.1
• Coordinates: 1805424..1805792 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA19_RS08545	1800357..1801523	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
FYA19_RS08550	1801642..1802115	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
FYA19_RS08555	1802313..1803371	+	1059	WP_001200891.1	FUSC family protein	-
FYA19_RS08560	1803543..1803872	+	330	WP_000450409.1	DUF496 family protein	-
FYA19_RS08565	1803973..1804155	-	183	WP_001016348.1	hypothetical protein	-
FYA19_RS08570	1804227..1804355	+	129	Protein_1673	transposase domain-containing protein	-
FYA19_RS08575	1804644..1804757	-	114	WP_001161660.1	DUF957 domain-containing protein	-
FYA19_RS08580	1804770..1804964	-	195	WP_000988600.1	hypothetical protein	-
FYA19_RS08585	1804961..1805335	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FYA19_RS08590	1805424..1805792	-	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FYA19_RS08595	1805866..1806087	-	222	WP_000692323.1	DUF987 domain-containing protein	-
FYA19_RS08600	1806150..1806596	-	447	WP_000187523.1	RadC family protein	-
FYA19_RS08605	1806593..1808125	-	1533	WP_001350525.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T137401 WP_000854814.1 NZ_CP043183:c1805335-1804961 [Escherichia coli O16:H48]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T137401 NZ_CP043183:c1805335-1804961 [Escherichia coli O16:H48]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT137401 WP_001285584.1 NZ_CP043183:c1805792-1805424 [Escherichia coli O16:H48]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT137401 NZ_CP043183:c1805792-1805424 [Escherichia coli O16:H48]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6