Detailed information of TA system TA137384

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4520217..4521012
• Replicon: chromosome
• Accession: NZ_CP043181
• Organism: Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: FYA20_RS21855
• Protein ID: WP_001094434.1
• Coordinates: 4520638..4521012 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: FYA20_RS21850
• Protein ID: WP_001285482.1
• Coordinates: 4520217..4520591 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA20_RS21820	4517097..4517918	+	822	WP_148886024.1	DUF945 domain-containing protein	-
FYA20_RS21830	4518255..4518728	+	474	WP_001313575.1	antirestriction protein	-
FYA20_RS21835	4518744..4519220	+	477	WP_001313574.1	RadC family protein	-
FYA20_RS21840	4519283..4519504	+	222	WP_000692315.1	DUF987 domain-containing protein	-
FYA20_RS21845	4519523..4520167	+	645	WP_000086762.1	hypothetical protein	-
FYA20_RS21850	4520217..4520591	+	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FYA20_RS21855	4520638..4521012	+	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
FYA20_RS21860	4521009..4521401	+	393	Protein_4183	hypothetical protein	-
FYA20_RS21865	4521442..4521639	+	198	WP_000839257.1	DUF957 domain-containing protein	-
FYA20_RS21870	4521732..4522574	+	843	WP_001280460.1	DUF4942 domain-containing protein	-
FYA20_RS21880	4523045..4523983	+	939	WP_000351277.1	glyoxylate/hydroxypyruvate reductase GhrA	-
FYA20_RS21885	4524038..4524775	+	738	WP_000283656.1	zinc-binding phosphatase	-
FYA20_RS21890	4524799..4525353	+	555	WP_001001913.1	molecular chaperone YcdY	-
FYA20_RS21895	4525455..4525946	+	492	WP_001300785.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T137384 WP_001094434.1 NZ_CP043181:4520638-4521012 [Escherichia coli O2:H6]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T137384 NZ_CP043181:4520638-4521012 [Escherichia coli O2:H6]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT137384 WP_001285482.1 NZ_CP043181:4520217-4520591 [Escherichia coli O2:H6]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT137384 NZ_CP056712:103760-103999 [Enterobacter asburiae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: Q1RDD2

Antitoxin

• AlphaFold DB: A0A0P7R0L9