Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3874033..3874651 Replicon chromosome
Accession NZ_CP043181
Organism Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FYA20_RS18685 Protein ID WP_001291435.1
Coordinates 3874033..3874251 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FYA20_RS18690 Protein ID WP_000344800.1
Coordinates 3874277..3874651 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA20_RS18650 3869320..3869892 + 573 WP_000779831.1 YbaY family lipoprotein -
FYA20_RS18655 3869923..3870234 - 312 WP_000409908.1 MGMT family protein -
FYA20_RS18665 3870613..3870966 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FYA20_RS18670 3871008..3872558 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FYA20_RS18675 3872722..3873192 - 471 WP_000136192.1 YlaC family protein -
FYA20_RS18680 3873308..3873859 - 552 WP_000102539.1 maltose O-acetyltransferase -
FYA20_RS18685 3874033..3874251 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FYA20_RS18690 3874277..3874651 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FYA20_RS18695 3875196..3878345 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
FYA20_RS18700 3878368..3879561 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T137383 WP_001291435.1 NZ_CP043181:c3874251-3874033 [Escherichia coli O2:H6]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T137383 NZ_CP043181:c3874251-3874033 [Escherichia coli O2:H6]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT137383 WP_000344800.1 NZ_CP043181:c3874651-3874277 [Escherichia coli O2:H6]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT137383 NZ_CP043181:c3874651-3874277 [Escherichia coli O2:H6]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References