Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3445833..3446383 Replicon chromosome
Accession NZ_CP043181
Organism Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag FYA20_RS16695 Protein ID WP_001160966.1
Coordinates 3446069..3446383 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag FYA20_RS16690 Protein ID WP_000125566.1
Coordinates 3445833..3446066 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA20_RS16665 3440906..3441193 + 288 WP_000203741.1 ferredoxin-like protein FixX -
FYA20_RS16670 3441252..3442583 + 1332 WP_148885965.1 MFS transporter -
FYA20_RS16675 3442691..3443221 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
FYA20_RS16680 3443214..3445076 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
FYA20_RS16685 3445268..3445747 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
FYA20_RS16690 3445833..3446066 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
FYA20_RS16695 3446069..3446383 + 315 WP_001160966.1 CcdB family protein Toxin
FYA20_RS16700 3446380..3447228 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
FYA20_RS16705 3447235..3447612 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
FYA20_RS16710 3447615..3448436 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
FYA20_RS16715 3448433..3449422 - 990 WP_000241228.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
FYA20_RS16720 3449422..3450708 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T137381 WP_001160966.1 NZ_CP043181:3446069-3446383 [Escherichia coli O2:H6]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T137381 NZ_CP056711:3748363-3748659 [Enterobacter asburiae]
GTGCCGGTTTCGTCACGCCTGTCGCCCGTAATGGTGTGGCAGCAGTTGTTAACGTATCTGCTGGAGCACCACTACGGCCT
CACACTCAACGACACACCATTTCATGACGACACGGCCATTGAGGAGCATATCGATGCGGGGATAACGCTTGCCGATGCTG
TTAATTTCCTGGTCGAAAAGTACGAACTGGCTCGTATCGACCGCAAGGGATTTACATGGCGAGAGCAACCCCCCTTCATT
ACTGCAACGGATATTCTGCGTGCCAAAAAGAACATCGGGCTGTTGCCCAGATCTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT137381 WP_000125566.1 NZ_CP043181:3445833-3446066 [Escherichia coli O2:H6]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT137381 NZ_CP043181:3445833-3446066 [Escherichia coli O2:H6]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References