Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3069454..3069951 Replicon chromosome
Accession NZ_CP043181
Organism Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FYA20_RS14835 Protein ID WP_001173343.1
Coordinates 3069778..3069951 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag FYA20_RS14830 Protein ID WP_001312331.1
Coordinates 3069454..3069750 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA20_RS14810 3066183..3066902 - 720 WP_000611288.1 two-component system response regulator DcuR -
FYA20_RS14815 3066899..3068530 - 1632 WP_001216466.1 sensor histidine kinase -
FYA20_RS14820 3068711..3068941 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
FYA20_RS14825 3068953..3069225 + 273 WP_000405647.1 N-acetyltransferase -
FYA20_RS14830 3069454..3069750 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FYA20_RS14835 3069778..3069951 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FYA20_RS14840 3070070..3071587 - 1518 WP_000003805.1 lysine--tRNA ligase -
FYA20_RS14845 3071824..3073281 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3069454..3078805 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T137377 WP_001173343.1 NZ_CP043181:3069778-3069951 [Escherichia coli O2:H6]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T137377 NZ_CP043181:3069778-3069951 [Escherichia coli O2:H6]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT137377 WP_001312331.1 NZ_CP043181:3069454-3069750 [Escherichia coli O2:H6]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT137377 NZ_CP056711:c1191504-1191130 [Enterobacter asburiae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References