Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1521399..1522053 Replicon chromosome
Accession NZ_CP043181
Organism Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag FYA20_RS07280 Protein ID WP_000244781.1
Coordinates 1521399..1521806 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FYA20_RS07285 Protein ID WP_000354046.1
Coordinates 1521787..1522053 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA20_RS07260 1517356..1519089 - 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -
FYA20_RS07265 1519095..1519805 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FYA20_RS07270 1519830..1520726 - 897 WP_000806633.1 site-specific tyrosine recombinase XerD -
FYA20_RS07275 1520838..1521359 + 522 WP_001055874.1 flavodoxin FldB -
FYA20_RS07280 1521399..1521806 - 408 WP_000244781.1 protein YgfX Toxin
FYA20_RS07285 1521787..1522053 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FYA20_RS07290 1522296..1523276 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
FYA20_RS07295 1523353..1524012 - 660 WP_000250274.1 hemolysin III family protein -
FYA20_RS07300 1524176..1524487 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
FYA20_RS07305 1524532..1525965 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T137372 WP_000244781.1 NZ_CP043181:c1521806-1521399 [Escherichia coli O2:H6]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T137372 NZ_CP056707:c3905942-3905601 [Enterobacter hormaechei]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGGATCTCGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTCGGTACGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT137372 WP_000354046.1 NZ_CP043181:c1522053-1521787 [Escherichia coli O2:H6]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT137372 NZ_CP043181:c1522053-1521787 [Escherichia coli O2:H6]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References