Detailed information of TA system TA137369

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 563957..564788
• Replicon: chromosome
• Accession: NZ_CP043181
• Organism: Escherichia coli O2:H6 strain PG20180057

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: FYA20_RS02820
• Protein ID: WP_000854814.1
• Coordinates: 564414..564788 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1PWQ3
• Locus tag: FYA20_RS02815
• Protein ID: WP_001285586.1
• Coordinates: 563957..564325 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYA20_RS02775	559014..560084	+	1071	WP_000102631.1	patatin-like phospholipase family protein	-
FYA20_RS02780	560220..560897	+	678	WP_001362823.1	hypothetical protein	-
FYA20_RS02785	560913..561323	+	411	WP_000846704.1	hypothetical protein	-
FYA20_RS02795	561544..562365	+	822	WP_001234571.1	DUF945 domain-containing protein	-
FYA20_RS02800	562628..563101	+	474	WP_000855074.1	antirestriction protein	-
FYA20_RS02805	563117..563593	+	477	WP_001351157.1	RadC family protein	-
FYA20_RS02810	563662..563883	+	222	WP_000692323.1	DUF987 domain-containing protein	-
FYA20_RS02815	563957..564325	+	369	WP_001285586.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FYA20_RS02820	564414..564788	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FYA20_RS02825	564785..564979	+	195	WP_000988601.1	hypothetical protein	-
FYA20_RS02830	564992..565105	+	114	WP_001161660.1	DUF957 domain-containing protein	-
FYA20_RS02835	565394..565540	-	147	Protein_532	transposase domain-containing protein	-
FYA20_RS02840	565594..565776	+	183	WP_001016348.1	hypothetical protein	-
FYA20_RS02845	565877..566206	-	330	WP_000450409.1	DUF496 family protein	-
FYA20_RS02850	566378..567436	-	1059	WP_001200890.1	FUSC family protein	-
FYA20_RS02855	567634..568107	-	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
FYA20_RS02860	568226..569392	-	1167	WP_001519880.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T137369 WP_000854814.1 NZ_CP043181:564414-564788 [Escherichia coli O2:H6]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T137369 NZ_CP056707:c2684864-2684761 [Enterobacter hormaechei]
GGCAGGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13563.41 Da
• Isoelectric Point: 5.0468

>AT137369 WP_001285586.1 NZ_CP043181:563957-564325 [Escherichia coli O2:H6]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT137369 NZ_CP043181:563957-564325 [Escherichia coli O2:H6]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: S1PWQ3