Detailed information of TA system TA137356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1630719..1631315
• Replicon: chromosome
• Accession: NZ_CP043179
• Organism: Pseudomonas protegens strain SN15-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q4KIY8
• Locus tag: FQ342_RS07665
• Protein ID: WP_011059021.1
• Coordinates: 1630719..1631021 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q4KIY9
• Locus tag: FQ342_RS07670
• Protein ID: WP_011059020.1
• Coordinates: 1631025..1631315 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FQ342_RS07645	1625953..1627266	-	1314	WP_041774047.1	precorrin-3B synthase	-
FQ342_RS07650	1627405..1628619	+	1215	WP_011059024.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
FQ342_RS07655	1628713..1629810	+	1098	WP_011059023.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
FQ342_RS07660	1629807..1630535	+	729	WP_011059022.1	cobalt-precorrin-6A reductase	-
FQ342_RS07665	1630719..1631021	+	303	WP_011059021.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FQ342_RS07670	1631025..1631315	+	291	WP_011059020.1	putative addiction module antidote protein	Antitoxin
FQ342_RS07675	1631416..1631814	+	399	WP_011059019.1	DUF2946 domain-containing protein	-
FQ342_RS07680	1631879..1632358	+	480	WP_026020078.1	copper chaperone PCu(A)C	-
FQ342_RS07685	1632414..1632824	+	411	WP_011059017.1	DUF2946 domain-containing protein	-
FQ342_RS07690	1632913..1635045	+	2133	WP_011059016.1	TonB-dependent copper receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-97)

Antitoxin

• Uncharacterized protein HI1420 (3-94)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11499.32 Da
• Isoelectric Point: 10.4481

>T137356 WP_011059021.1 NZ_CP043179:1630719-1631021 [Pseudomonas protegens]
MFVFEQTPEFSRWLLNLKDPQGKTRVLARIRAAQLGRFGDCEPVGEGVHEIRIHRGPGYRVYFSRHGKMIYLLLIGGDKA
TQKRDIQRARQLACDLKSEG

Nucleotide

• Length: 303 bp

>T137356 NZ_CP043179:1630719-1631021 [Pseudomonas protegens]
ATGTTCGTTTTCGAGCAGACTCCTGAGTTCAGCCGCTGGCTGCTGAACCTGAAGGACCCGCAAGGGAAAACCCGTGTACT
GGCCAGGATCCGCGCCGCACAGCTGGGCCGTTTTGGCGACTGCGAACCTGTGGGCGAAGGCGTCCACGAGATACGCATCC
ACCGCGGCCCGGGTTACCGGGTGTACTTCTCGCGCCACGGCAAAATGATCTACCTGCTGTTGATCGGCGGCGACAAGGCC
ACCCAGAAACGCGATATCCAACGGGCCAGGCAACTGGCCTGCGACCTCAAGAGCGAGGGCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10423.94 Da
• Isoelectric Point: 5.0939

>AT137356 WP_011059020.1 NZ_CP043179:1631025-1631315 [Pseudomonas protegens]
MRTPVTRFDAAEYLKTPEDMVAYLDACFEEDTGDGVLIRAALNDIARARGMTQVARDTGLGRESLYKALGSQGNPEFATI
IKVMKALGLKINVSNA

Nucleotide

• Length: 291 bp

>AT137356 NZ_CP056704:37141-37410 [Klebsiella grimontii]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACGATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGCCGCT
TGATTCTCAGTGAAGAGTCCTGGAATCTGGTAATGGATGCCATCAGTAACCCGCCTGCACCGAACGATAGGCTGAAACGA
GCTGCCAAACGTCTACAAAGCATGGAGTAA

Structures

Toxin

• AlphaFold DB: Q4KIY8

Antitoxin

• AlphaFold DB: Q4KIY9