Detailed information of TA system TA137133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 4530137..4530668
• Replicon: chromosome
• Accession: NZ_CP043083
• Organism: Bordetella parapertussis strain J293

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: FYB40_RS21335
• Protein ID: WP_003812626.1
• Coordinates: 4530137..4530403 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: FYB40_RS21340
• Protein ID: WP_003816743.1
• Coordinates: 4530390..4530668 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FYB40_RS21310	4525264..4525536	+	273	WP_003812615.1	hypothetical protein	-
FYB40_RS21315	4525575..4526285	-	711	WP_003812617.1	aquaporin Z	-
FYB40_RS21320	4526396..4527028	-	633	WP_003812619.1	PAS domain-containing protein	-
FYB40_RS21325	4527425..4528303	+	879	WP_041937387.1	tripartite tricarboxylate transporter substrate binding protein	-
FYB40_RS21330	4528365..4529906	+	1542	WP_010928296.1	M81 family metallopeptidase	-
FYB40_RS21335	4530137..4530403	+	267	WP_003812626.1	BrnT family toxin	Toxin
FYB40_RS21340	4530390..4530668	+	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
FYB40_RS21350	4530962..4531618	-	657	WP_010928295.1	phosphoribosylanthranilate isomerase	-
FYB40_RS21355	4531633..4533300	-	1668	WP_010928294.1	TRAP transporter large permease subunit	-
FYB40_RS21360	4533303..4533932	-	630	WP_010928293.1	TRAP transporter small permease subunit	-
FYB40_RS21365	4534144..4535238	-	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T137133 WP_003812626.1 NZ_CP043083:4530137-4530403 [Bordetella parapertussis]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T137133 NZ_CP056515:4503208-4503495 [Citrobacter freundii]
ATGAGTTATACGGTAAAATTCAGGGAAGATGCGCTGAAAGAATGGCAAAAACTGGATAAAACCATTCAGCAGCAGCTTGC
CAAAGAACTTAAGAAATGCGGTGAAAATCCGCATGTCCCCTCCGCGAAGCTCAGGGGAATAAAAGATTGCTATAAAATCC
AACTACGCACTTCAAGGTTCCGTCTTGTTTATCAAGTTATTGACGACACCCTGGTCATCGCTGTCGTTGCCGTCGGTAAA
CGCGAGCGTAGTGAAGTTTATAATCTTGCCAGCGAAAGACTACGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT137133 WP_003816743.1 NZ_CP043083:4530390-4530668 [Bordetella parapertussis]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT137133 NZ_CP056515:4502960-4503211 [Citrobacter freundii]
ATGCCACATATCATTTTAAGCGACACCACCGCCAGCGTGAGTGAGCTAAAAAAGAACCCGATGGCGACTGTTAGCGCAGG
GGATGGGTTTCCGGTCGCAATCCTGAACCGTAACCAGCCCGCCTTTTATTGTGTTCCCGCTGAGCTTTACGAAAAAATGC
TTGATGCGCTGGACGATCAGCAACTGGTTAAATTAGTCCATGAACGGAGCCAACAGCCACTGGTGGATGTTGATCTGGAC
AGCTATTTATGA

Structures

Toxin

• AlphaFold DB: K0MH61

Antitoxin

• AlphaFold DB: K0MF02