Detailed information of TA system TA137034

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 18510..19402
• Replicon: plasmid pUHCC0315a
• Accession: NZ_CP043057
• Organism: Dolichospermum sp. UHCC 0315A

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: BMF77_RS25495
• Protein ID: WP_148766665.1
• Coordinates: 18510..18953 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: BMF77_RS25500
• Protein ID: WP_148766667.1
• Coordinates: 18950..19402 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMF77_RS25485	14019..15062	-	1044	WP_148766661.1	type I-D CRISPR-associated protein Cas7/Csc2	-
BMF77_RS25490	15155..18517	-	3363	WP_148766663.1	type I-D CRISPR-associated protein Cas10d/Csc3	-
BMF77_RS25495	18510..18953	-	444	WP_148766665.1	DUF86 domain-containing protein	Toxin
BMF77_RS25500	18950..19402	-	453	WP_148766667.1	nucleotidyltransferase domain-containing protein	Antitoxin
BMF77_RS25505	19507..19713	-	207	WP_148766669.1	hypothetical protein	-
BMF77_RS25510	20157..21476	-	1320	Protein_17	type I-D CRISPR-associated helicase Cas3'	-
BMF77_RS25515	21543..22597	+	1055	Protein_18	IS630 family transposase	-
BMF77_RS25520	22652..22975	+	324	Protein_19	DUF4277 domain-containing protein	-
BMF77_RS25525	23013..24067	-	1055	Protein_20	IS630 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..96277	96277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (17-133)

Antitoxin

• Polymerase beta, nucleotidyltransferase (16-112)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16982.40 Da
• Isoelectric Point: 4.5034

>T137034 WP_148766665.1 NZ_CP043057:c18953-18510 [Dolichospermum sp. UHCC 0315A]
MNNLDPEIVLARLKLIAKYYNTLEEFRSISLDEFLADFRQQLIVERLLQLMTQAAIDINDHILSKLNSGKSYTNFEAFIE
LGNSQIITPELAKQIAPSSGLRNRLVHEYDDIDPKQVFMAINFALQQYPLYVRQINSYLISLEEDNG

Nucleotide

• Length: 444 bp

>T137034 NZ_CP056423:c11716-11534 [Citrobacter sp. RHBSTW-00524]
GTGAAATATTCGGAGTTCAGACGCTGGCTAGTTCAGCAGGGGGCCGAGTTCAAAAAGGCACCAGGAGGCGGAAGCCACCA
AAAGGTCAGTTTGAACGGTATGAGGTCAATTTTTCCTGACCACGGTTCTAAAGAAATGCCAGAACCACTAAGGAAAAAAA
TAATGAAGGACCTGGGGCTTTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 17446.92 Da
• Isoelectric Point: 5.0206

>AT137034 WP_148766667.1 NZ_CP043057:c19402-18950 [Dolichospermum sp. UHCC 0315A]
MENNTPTITELQKLSSQFTEKIPYLKMLVLFGSRATDKTNINSDWDFAVLCDEKQRQSYIENNMSGLFELPILIGEVLKI
NPDIIDTVELNQCSWLIAHFVARDGISLFEKHPGEFDNFRLNSVKTQSELKKFRQEQHRIIEIELNKWGA

Nucleotide

• Length: 453 bp

>AT137034 NZ_CP043057:c19402-18950 [Dolichospermum sp. UHCC 0315A]
ATGGAAAACAACACTCCCACAATTACAGAACTTCAAAAACTATCCTCACAATTTACTGAAAAAATACCTTACTTAAAAAT
GTTAGTCCTGTTTGGTTCTAGAGCTACTGACAAAACTAATATAAATAGTGACTGGGATTTTGCAGTTTTGTGTGATGAAA
AACAACGTCAATCTTACATAGAAAATAATATGTCTGGTTTATTTGAACTGCCAATTTTGATTGGTGAAGTTTTGAAAATA
AATCCTGATATCATAGATACTGTCGAATTAAATCAATGTTCTTGGTTAATTGCTCATTTTGTTGCGCGTGACGGAATTAG
TTTATTTGAAAAACACCCTGGAGAATTTGATAACTTCCGCTTAAATTCAGTAAAAACTCAATCAGAGTTAAAAAAATTCC
GCCAAGAACAACACCGAATTATTGAAATAGAACTAAATAAGTGGGGAGCATGA