Detailed information of TA system TA137032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4976322..4976728
• Replicon: chromosome
• Accession: NZ_CP043056
• Organism: Dolichospermum sp. UHCC 0315A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BMF77_RS23570
• Protein ID: WP_148766281.1
• Coordinates: 4976322..4976513 (+)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BMF77_RS23575
• Protein ID: WP_148766283.1
• Coordinates: 4976513..4976728 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMF77_RS23550	4972662..4973558	+	897	WP_148766273.1	1-aminocyclopropane-1-carboxylate deaminase/D-cysteine desulfhydrase	-
BMF77_RS23555	4973555..4973815	-	261	WP_148766275.1	hypothetical protein	-
BMF77_RS23560	4973890..4974474	-	585	WP_148766277.1	Uma2 family endonuclease	-
BMF77_RS23565	4974771..4976192	-	1422	WP_148766279.1	pentapeptide repeat-containing protein	-
BMF77_RS23570	4976322..4976513	+	192	WP_148766281.1	type II toxin-antitoxin system HicA family toxin	Toxin
BMF77_RS23575	4976513..4976728	+	216	WP_148766283.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BMF77_RS23580	4976771..4979413	-	2643	WP_148766285.1	alanine--tRNA ligase	-
BMF77_RS23585	4979600..4979824	+	225	WP_148766287.1	type II toxin-antitoxin system HicA family toxin	-
BMF77_RS23590	4979821..4980048	+	228	WP_148766288.1	type II toxin-antitoxin system HicB family antitoxin	-
BMF77_RS23595	4980084..4980449	-	366	WP_210422256.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-60)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7204.45 Da
• Isoelectric Point: 10.3448

>T137032 WP_148766281.1 NZ_CP043056:4976322-4976513 [Dolichospermum sp. UHCC 0315A]
MKVKEVLKILEEDGWYIDRIRGSHRILKHQIKSGIVVLAGKLSKDLAEGTRKSIFSQAQLEDK

Nucleotide

• Length: 192 bp

>T137032 NZ_CP043056:4976322-4976513 [Dolichospermum sp. UHCC 0315A]
ATGAAGGTTAAAGAAGTTCTCAAAATCCTGGAAGAAGATGGTTGGTATATAGATCGCATTAGAGGTAGTCATCGTATCCT
CAAACATCAAATTAAATCGGGTATCGTGGTATTAGCGGGTAAACTTAGTAAAGACTTAGCGGAAGGGACTCGAAAGAGTA
TTTTTTCCCAAGCACAACTGGAGGACAAATAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7811.91 Da
• Isoelectric Point: 3.9488

>AT137032 WP_148766283.1 NZ_CP043056:4976513-4976728 [Dolichospermum sp. UHCC 0315A]
MKNYAVVYEWAGSNYSAYVPDLPGCVACGDTLQETEELIKEAIELYLEVLKEDGKPIPEAFTQVGKVAVTI

Nucleotide

• Length: 216 bp

>AT137032 NZ_CP043056:4976513-4976728 [Dolichospermum sp. UHCC 0315A]
ATGAAAAATTATGCAGTAGTCTATGAATGGGCAGGTAGTAATTATTCTGCTTATGTTCCAGATTTACCCGGATGTGTTGC
TTGCGGTGATACCTTACAAGAAACAGAAGAACTAATTAAAGAAGCTATAGAATTGTATTTGGAAGTATTGAAAGAAGATG
GAAAACCTATACCTGAAGCTTTTACACAAGTAGGTAAAGTAGCAGTGACTATATAA