Detailed information of TA system TA137029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 2518423..2519000
• Replicon: chromosome
• Accession: NZ_CP043056
• Organism: Dolichospermum sp. UHCC 0315A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BMF77_RS12210
• Protein ID: WP_148763104.1
• Coordinates: 2518423..2518683 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BMF77_RS12215
• Protein ID: WP_148763106.1
• Coordinates: 2518671..2519000 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMF77_RS12185	2515387..2516475	+	1089	WP_148763094.1	hypothetical protein	-
BMF77_RS12190	2516529..2516921	-	393	WP_148763096.1	type II toxin-antitoxin system VapC family toxin	-
BMF77_RS12195	2516911..2517138	-	228	WP_148763098.1	hypothetical protein	-
BMF77_RS12200	2517524..2518009	-	486	WP_148763100.1	DUF3368 domain-containing protein	-
BMF77_RS12205	2518015..2518254	-	240	WP_148763102.1	UPF0175 family protein	-
BMF77_RS12210	2518423..2518683	+	261	WP_148763104.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
BMF77_RS12215	2518671..2519000	+	330	WP_148763106.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
BMF77_RS12220	2519121..2521901	-	2781	WP_148763109.1	preprotein translocase subunit SecA	-
BMF77_RS12225	2522043..2522579	-	537	Protein_2376	cytochrome C	-
BMF77_RS12230	2522714..2523478	-	765	WP_148763111.1	flagellar motor protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (10-52)

Antitoxin

• mRNA interferase PemK-like (7-107)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9955.34 Da
• Isoelectric Point: 4.8430

>T137029 WP_148763104.1 NZ_CP043056:2518423-2518683 [Dolichospermum sp. UHCC 0315A]
MGTAIKTRIVKIGNSQGLRIPKTLLEQSGINSEVEIEVHGNHLIIRPVTQIRKGWEKAFIEMAEKQDDVLLDDINTTEWD
KDEWQW

Nucleotide

• Length: 261 bp

>T137029 NZ_CP043056:2518423-2518683 [Dolichospermum sp. UHCC 0315A]
ATGGGTACAGCAATTAAAACCAGAATAGTCAAAATCGGCAATTCCCAAGGTTTACGCATTCCCAAAACCTTATTAGAACA
AAGCGGAATTAACTCAGAAGTAGAAATAGAAGTTCACGGTAATCATCTGATTATTCGCCCAGTTACACAAATAAGAAAAG
GTTGGGAAAAAGCATTTATAGAAATGGCAGAAAAACAAGATGATGTCCTATTAGATGATATCAACACAACAGAATGGGAT
AAAGATGAATGGCAATGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12235.33 Da
• Isoelectric Point: 7.1338

>AT137029 WP_148763106.1 NZ_CP043056:2518671-2519000 [Dolichospermum sp. UHCC 0315A]
MAMVVNRFDVFLVNLDPTIGSEIKKTRPCLIISPNEINHHIATVIVAPMTTKGQPYPTRVTCQFQGQNGQIVLDQIRTVD
KTRLVKLLGQITTEEQKTVLDILAEMFAE

Nucleotide

• Length: 330 bp

>AT137029 NZ_CP043056:2518671-2519000 [Dolichospermum sp. UHCC 0315A]
ATGGCAATGGTAGTTAACAGATTTGATGTTTTCCTAGTTAATCTTGACCCTACCATTGGGAGTGAAATTAAAAAAACACG
CCCCTGCTTAATAATTTCACCAAACGAGATAAACCACCATATTGCCACCGTCATAGTTGCACCAATGACAACAAAAGGGC
AACCATACCCCACAAGAGTAACCTGTCAATTTCAAGGACAAAATGGACAAATTGTATTAGACCAAATTCGCACAGTAGAT
AAAACTCGATTAGTCAAACTTCTGGGTCAAATTACTACAGAAGAACAAAAAACAGTTCTGGATATCCTAGCTGAAATGTT
CGCCGAATAA