Detailed information of TA system TA137021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 185368..185952
• Replicon: plasmid pAP43-OXA58-NDM1
• Accession: NZ_CP043053
• Organism: Acinetobacter pittii strain AP43

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: FXO17_RS19990
• Protein ID: WP_000286964.1
• Coordinates: 185368..185670 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FXO17_RS19995
• Protein ID: WP_000985609.1
• Coordinates: 185671..185952 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXO17_RS19950	180563..181126	-	564	WP_032073152.1	recombinase family protein	-
FXO17_RS19955	181185..181889	+	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
FXO17_RS19960	181943..182476	+	534	Protein_195	IS6-like element IS1006 family transposase	-
FXO17_RS19965	182604..182942	-	339	WP_032073153.1	hypothetical protein	-
FXO17_RS19970	183001..183771	+	771	WP_032073134.1	hypothetical protein	-
FXO17_RS19975	184012..184299	+	288	WP_000438825.1	BrnT family toxin	-
FXO17_RS19980	184286..184600	+	315	WP_004728120.1	BrnA antitoxin family protein	-
FXO17_RS19985	184689..185219	-	531	WP_000172759.1	hypothetical protein	-
FXO17_RS19990	185368..185670	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FXO17_RS19995	185671..185952	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
FXO17_RS20000	186032..186451	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
FXO17_RS20005	186591..186911	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FXO17_RS20010	186904..187176	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
FXO17_RS20015	187669..189144	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
FXO17_RS20020	189200..190084	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VIa / aac(3)-IId / blaOXA-58 / msr(E) / mph(E) / floR	-	1..268263	268263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T137021 WP_000286964.1 NZ_CP043053:185368-185670 [Acinetobacter pittii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T137021 NZ_CP056409:4865556-4865867 [Citrobacter sp. RHBSTW-00535]
ATGCATGTTATCAGCCGAAAACCTTTCAACGAGGCAAGACTCCGTTTTCCAAACCACATGGCAGCACTCGTTGATTTGCT
GAATATTTTAGAGAAAAAAATGTTCCACACTCCTGAAGAGATGAAGCAGTACATACCTTCACTGGATAATTTTAAATACA
GAAATAAATGGTGGGTGATTAATGTTTCAGGTAACTGCTTGAGACTTATTGCCTATATTGATTTCAAACTGCAAAAGGTA
TTTGTGAAGCATATCGTTCACCATGCCGAGTATGACAGACTAACCACATACTATCGGGGTCACAAAGAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT137021 WP_000985609.1 NZ_CP043053:185671-185952 [Acinetobacter pittii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT137021 NZ_CP043053:185671-185952 [Acinetobacter pittii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94