Detailed information of TA system TA137020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 184012..184600
• Replicon: plasmid pAP43-OXA58-NDM1
• Accession: NZ_CP043053
• Organism: Acinetobacter pittii strain AP43

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: FXO17_RS19975
• Protein ID: WP_000438825.1
• Coordinates: 184012..184299 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: FXO17_RS19980
• Protein ID: WP_004728120.1
• Coordinates: 184286..184600 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXO17_RS19945	179493..180311	-	819	WP_032073151.1	AraC family transcriptional regulator	-
FXO17_RS19950	180563..181126	-	564	WP_032073152.1	recombinase family protein	-
FXO17_RS19955	181185..181889	+	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
FXO17_RS19960	181943..182476	+	534	Protein_195	IS6-like element IS1006 family transposase	-
FXO17_RS19965	182604..182942	-	339	WP_032073153.1	hypothetical protein	-
FXO17_RS19970	183001..183771	+	771	WP_032073134.1	hypothetical protein	-
FXO17_RS19975	184012..184299	+	288	WP_000438825.1	BrnT family toxin	Toxin
FXO17_RS19980	184286..184600	+	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
FXO17_RS19985	184689..185219	-	531	WP_000172759.1	hypothetical protein	-
FXO17_RS19990	185368..185670	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FXO17_RS19995	185671..185952	+	282	WP_000985609.1	putative addiction module antidote protein	-
FXO17_RS20000	186032..186451	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
FXO17_RS20005	186591..186911	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FXO17_RS20010	186904..187176	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
FXO17_RS20015	187669..189144	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VIa / aac(3)-IId / blaOXA-58 / msr(E) / mph(E) / floR	-	1..268263	268263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T137020 WP_000438825.1 NZ_CP043053:184012-184299 [Acinetobacter pittii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T137020 NZ_CP043053:184012-184299 [Acinetobacter pittii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT137020 WP_004728120.1 NZ_CP043053:184286-184600 [Acinetobacter pittii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT137020 NZ_CP043053:184286-184600 [Acinetobacter pittii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9