Detailed information of TA system TA137017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 131159..131895
• Replicon: plasmid pKLP268-3
• Accession: NZ_CP043049
• Organism: Klebsiella pneumoniae strain KLP268

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: FXN60_RS28915
• Protein ID: WP_003026803.1
• Coordinates: 131159..131641 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: FXN60_RS28920
• Protein ID: WP_003026799.1
• Coordinates: 131629..131895 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FXN60_RS28900	126796..127758	+	963	WP_004152113.1	M48 family metalloprotease	-
FXN60_RS28905	127846..129100	+	1255	Protein_130	IS3 family transposase	-
FXN60_RS28910	129601..130947	-	1347	WP_020314316.1	ISNCY family transposase	-
FXN60_RS28915	131159..131641	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
FXN60_RS28920	131629..131895	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
FXN60_RS28925	132071..132325	-	255	WP_004152108.1	hypothetical protein	-
FXN60_RS28930	132401..132658	-	258	WP_004152107.1	hypothetical protein	-
FXN60_RS28935	132707..132910	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
FXN60_RS28940	132944..133312	-	369	WP_004152105.1	hypothetical protein	-
FXN60_RS28945	133356..133850	-	495	WP_004152104.1	DNA-binding protein	-
FXN60_RS28950	133881..134456	-	576	WP_004152103.1	hypothetical protein	-
FXN60_RS28955	134444..134713	-	270	WP_004152102.1	hypothetical protein	-
FXN60_RS28960	135071..135421	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
FXN60_RS28965	135471..135833	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1 / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..212420	212420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T137017 WP_003026803.1 NZ_CP043049:c131641-131159 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T137017 NZ_CP043049:c131641-131159 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT137017 WP_003026799.1 NZ_CP043049:c131895-131629 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT137017 NZ_CP043049:c131895-131629 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66