Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 16588..17231 Replicon plasmid pKLP268-2
Accession NZ_CP043048
Organism Klebsiella pneumoniae strain KLP268

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag FXN60_RS27880 Protein ID WP_001044770.1
Coordinates 16588..17004 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag FXN60_RS27885 Protein ID WP_001261282.1
Coordinates 17001..17231 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FXN60_RS30345 12479..12544 + 66 Protein_16 EAL domain-containing protein -
FXN60_RS27845 12684..13448 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
FXN60_RS27855 13591..13857 - 267 WP_001144737.1 plasmid mobilization relaxosome protein MobC -
FXN60_RS27860 14078..14551 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
FXN60_RS27865 14707..15474 + 768 Protein_20 class 1 integron integrase IntI1 -
FXN60_RS27870 15527..16231 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
FXN60_RS27875 16278..16514 - 237 WP_001348075.1 AAA family ATPase -
FXN60_RS27880 16588..17004 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
FXN60_RS27885 17001..17231 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FXN60_RS27890 17188..17648 + 461 Protein_25 hypothetical protein -
FXN60_RS27895 17776..18096 + 321 WP_032072060.1 hypothetical protein -
FXN60_RS27900 18145..18783 + 639 WP_022644887.1 hypothetical protein -
FXN60_RS27905 18786..19571 + 786 WP_022644886.1 site-specific integrase -
FXN60_RS27910 19629..19886 - 258 WP_032408936.1 hypothetical protein -
FXN60_RS30350 20015..20128 - 114 WP_032072094.1 hypothetical protein -
FXN60_RS27920 20759..21514 + 756 WP_012539983.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / blaKPC-31 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 - 1..71836 71836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T137016 WP_001044770.1 NZ_CP043048:c17004-16588 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T137016 NZ_CP043048:c17004-16588 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT137016 WP_001261282.1 NZ_CP043048:c17231-17001 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT137016 NZ_CP043048:c17231-17001 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References